151912

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1733686..1734523 Replicon chromosome
Accession NZ_CP049967
Organism Escherichia coli strain ARL09/232

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag G9448_RS08525 Protein ID WP_000227784.1
Coordinates 1733981..1734523 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag G9448_RS08520 Protein ID WP_001297137.1
Coordinates 1733686..1733997 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9448_RS08495 1728706..1729653 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
G9448_RS08500 1729675..1731666 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
G9448_RS08505 1731656..1732270 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
G9448_RS08510 1732270..1732599 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
G9448_RS08515 1732611..1733501 + 891 WP_000971336.1 heme o synthase -
G9448_RS08520 1733686..1733997 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
G9448_RS08525 1733981..1734523 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
G9448_RS08530 1734579..1735514 - 936 WP_001365761.1 sel1 repeat family protein -
G9448_RS08535 1735922..1737286 + 1365 WP_001000974.1 MFS transporter -
G9448_RS08540 1737414..1737905 - 492 WP_001138904.1 nucleotide binding protein YajQ -
G9448_RS08545 1738073..1738984 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T151912 WP_000227784.1 NZ_CP049967:1733981-1734523 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T151912 NZ_CP065833:c3666552-3666235 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT151912 WP_001297137.1 NZ_CP049967:1733686-1733997 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT151912 NZ_CP065833:c3666242-3665955 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References