Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 719709..720347 Replicon chromosome
Accession NZ_CP049967
Organism Escherichia coli strain ARL09/232

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G9448_RS03615 Protein ID WP_000813794.1
Coordinates 720171..720347 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G9448_RS03610 Protein ID WP_001270286.1
Coordinates 719709..720125 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9448_RS03590 714861..715802 - 942 WP_001251315.1 ABC transporter permease -
G9448_RS03595 715803..716816 - 1014 WP_000220416.1 ABC transporter ATP-binding protein -
G9448_RS03600 716834..717979 - 1146 WP_000047455.1 ABC transporter substrate-binding protein -
G9448_RS03605 718224..719630 - 1407 WP_000760589.1 PLP-dependent aminotransferase family protein -
G9448_RS03610 719709..720125 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G9448_RS03615 720171..720347 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G9448_RS03620 720569..720799 + 231 WP_000494244.1 YncJ family protein -
G9448_RS03625 720891..722852 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G9448_RS03630 722925..723461 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
G9448_RS03635 723514..724728 + 1215 WP_001307190.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 724768..726033 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T151910 WP_000813794.1 NZ_CP049967:c720347-720171 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T151910 NZ_CP065833:1876061-1876163 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT151910 WP_001270286.1 NZ_CP049967:c720125-719709 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT151910 NZ_CP065833:c1876170-1876025 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References