Detailed information of TA system TA151901

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 100758..101589
• Replicon: chromosome
• Accession: NZ_CP049967
• Organism: Escherichia coli strain ARL09/232

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: G9448_RS00530
• Protein ID: WP_000854814.1
• Coordinates: 100758..101132 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: G9448_RS00535
• Protein ID: WP_001285585.1
• Coordinates: 101221..101589 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9448_RS00490	96154..97320	+	1167	WP_001442313.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
G9448_RS00495	97439..97912	+	474	WP_001105393.1	DNA gyrase inhibitor SbmC	-
G9448_RS00500	98110..99168	+	1059	Protein_99	FUSC family protein	-
G9448_RS00505	99340..99669	+	330	WP_000450409.1	DUF496 family protein	-
G9448_RS00510	99770..99952	-	183	WP_072769026.1	ethanolamine utilization protein	-
G9448_RS00515	100024..100152	+	129	Protein_102	transposase domain-containing protein	-
G9448_RS00520	100441..100554	-	114	WP_001161660.1	DUF957 domain-containing protein	-
G9448_RS00525	100567..100761	-	195	WP_000988600.1	hypothetical protein	-
G9448_RS00530	100758..101132	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
G9448_RS00535	101221..101589	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
G9448_RS00540	101663..101884	-	222	WP_000692323.1	DUF987 domain-containing protein	-
G9448_RS00545	101947..102423	-	477	WP_001186773.1	RadC family protein	-
G9448_RS00550	102439..102918	-	480	WP_000860076.1	antirestriction protein	-
G9448_RS00555	103000..103821	-	822	WP_001234530.1	DUF945 domain-containing protein	-
G9448_RS00560	104042..104452	-	411	WP_000846713.1	hypothetical protein	-
G9448_RS00565	104468..105151	-	684	WP_000775500.1	hypothetical protein	-
G9448_RS00570	105287..106357	-	1071	WP_000102643.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T151901 WP_000854814.1 NZ_CP049967:c101132-100758 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T151901 NZ_CP065831:4179533-4179751 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT151901 WP_001285585.1 NZ_CP049967:c101589-101221 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT151901 NZ_CP065831:4179132-4179506 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAGGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60