Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5336506..5337131 Replicon chromosome
Accession NZ_CP049953
Organism Klebsiella pneumoniae strain 19KM1109

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag G9175_RS26220 Protein ID WP_002882817.1
Coordinates 5336506..5336889 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag G9175_RS26225 Protein ID WP_004150355.1
Coordinates 5336889..5337131 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9175_RS26205 5333872..5334774 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
G9175_RS26210 5334771..5335406 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
G9175_RS26215 5335403..5336332 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
G9175_RS26220 5336506..5336889 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
G9175_RS26225 5336889..5337131 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
G9175_RS26230 5337336..5338253 + 918 WP_002882812.1 alpha/beta hydrolase -
G9175_RS26235 5338267..5339208 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
G9175_RS26240 5339253..5339690 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
G9175_RS26245 5339687..5340547 - 861 WP_002882807.1 virulence factor BrkB family protein -
G9175_RS26250 5340541..5341140 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T151876 WP_002882817.1 NZ_CP049953:c5336889-5336506 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T151876 NZ_CP065824:c2214723-2214151 [Pseudomonas tolaasii]
ATGGACATTGAAAAGATCAAAGGTCGCCTCGACTTTCTGCGCGAAGCGGAAAAACTCAAGGACGTGCTCAGAAGCGCCCA
CACGGCGTCCGGGCGCACCGAAAGCACGGCTGAACACAGCTGGCGCCTGTGCCTGATGGCGATGGTGTTCGCCGATGAAC
TGCCTGGGCTCGACCTGCTGAAAATCCTCAAAATGTGCGTCATCCATGACTTGGGCGAAGCCCTCCATGGCGACATCCCG
GCGGTCAGCCAGGCAGCATTTCCCAACAAGGGCGAGCAGGAGCGCAATGACCTGCTGGTTCTGGCCCAGCCCCTGGACCG
CCCCCTGCGCGATGAAATCCTGGCACTGTGGGACGACTACGAAAACGCAGCGTCACCCGAAGCCAGGGCCGTCAAGGCCA
TGGACAAGCTGGAAACCCTGCTGCAACACACCCAGGGGCTGAACCCGCCCGATTTCGACTACGCCTTCAACCTGGCTTAC
GGCAAAAAACACACGGATGCCGAACCAATCTTCGAGAGGCTGCGCCATCTCATCGACAAGGCCACTCAGGAGAAGCTCGA
CGCCACGCCTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT151876 WP_004150355.1 NZ_CP049953:c5337131-5336889 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT151876 NZ_CP065824:c2215361-2214804 [Pseudomonas tolaasii]
ATGCCCGCTCCATTGCTCAAGCCAGCCTTGCCAGGTATTGCTCTGCGGCGTTGGCGGACGCTGCACCGCGTCAAGCAAAG
CCATGCCGCGCAGATGTTCAAGGTGACCCAATCGACCATTTCGCGCTGGGAAAGCGGCGCACTGGCCATGGACCCCGATG
CGCACCACCAGCTTGAGCGTTTGTTGGCAGCGCGCCTTGAAGCGGCTGCCGATCATGCGCTGGCCCGCCTGGTCGGGGAG
AGTTCGCGCCCGGTGCATCTGGTGTGCGACCTGACCCATCGCCTGCTGGCGTGTTCGCCTTCCCGCGCCGCGCAATTCAG
CAGGCCGCTGGGCGAATTGATCGGGCAGTCGCTATGGCACTTCGCAACGCCTGAAATCGTGCAGGTTGAGGCCGCGCTAG
AAACCGCCGGTTGGCGCGAAACCCAGGCGCCACCGGCGCTGGAGTTCATGACCGGCCGCAACGCCTCCAGCCTCGTGCCC
ATTCACCCCGGCCTGTGCCGCTGGACGCGCCTGGTGTTGAGCGATGGTAGCGCCGCGCGCCTGGTCGAGACGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References