Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 26742..27385 Replicon plasmid pMBR_DHA-1_1CV3
Accession NZ_CP049952
Organism Klebsiella pneumoniae strain 19KM1053

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag G9174_RS27360 Protein ID WP_000754566.1
Coordinates 26969..27385 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag G9174_RS27355 Protein ID WP_001261276.1
Coordinates 26742..26972 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9174_RS27320 21861..22130 + 270 WP_000339857.1 hypothetical protein -
G9174_RS27325 22307..23173 - 867 WP_004118283.1 replication initiation protein -
G9174_RS27330 23703..23807 + 105 WP_032409716.1 hypothetical protein -
G9174_RS27335 23936..24193 + 258 WP_000764642.1 hypothetical protein -
G9174_RS27340 24251..25027 - 777 WP_000015958.1 site-specific integrase -
G9174_RS27345 25024..25767 - 744 WP_000129823.1 hypothetical protein -
G9174_RS27350 25818..26168 - 351 WP_000493378.1 hypothetical protein -
G9174_RS27355 26742..26972 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G9174_RS27360 26969..27385 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
G9174_RS27365 27589..28445 + 857 Protein_31 IS3-like element ISEc15 family transposase -
G9174_RS27370 28727..29803 + 1077 WP_000227969.1 IS110 family transposase -
G9174_RS27375 30444..30527 + 84 Protein_33 SOS response-associated peptidase -
G9174_RS27380 30690..31214 - 525 Protein_34 Tn3 family transposase -
G9174_RS27385 31279..31983 + 705 WP_001067834.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / mph(A) - 1..37432 37432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T151857 WP_000754566.1 NZ_CP049952:26969-27385 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T151857 NZ_CP065789:2273201-2273317 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT151857 WP_001261276.1 NZ_CP049952:26742-26972 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT151857 NZ_CP065789:c2273491-2273312 [Bacillus subtilis]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References