Detailed information of TA system TA151832

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4797272..4798107
• Replicon: chromosome
• Accession: NZ_CP049947
• Organism: Klebsiella pneumoniae strain 19KM267

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A5E2RWI0
• Locus tag: G9173_RS23725
• Protein ID: WP_001559913.1
• Coordinates: 4797272..4797649 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A5E3EVB5
• Locus tag: G9173_RS23730
• Protein ID: WP_032408847.1
• Coordinates: 4797739..4798107 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9173_RS23685	4792733..4793407	+	675	WP_004152211.1	hypothetical protein	-
G9173_RS23690	4793419..4793757	+	339	WP_064449948.1	hypothetical protein	-
G9173_RS23695	4793760..4794239	+	480	WP_002887049.1	type VI secretion system tube protein Hcp	-
G9173_RS23700	4794431..4794754	-	324	Protein_4649	superfamily I DNA helicase	-
G9173_RS23705	4794773..4795884	-	1112	Protein_4650	IS3 family transposase	-
G9173_RS23710	4796333..4796485	-	153	WP_164713811.1	hypothetical protein	-
G9173_RS23715	4796570..4796857	-	288	WP_073970535.1	DUF957 domain-containing protein	-
G9173_RS23720	4796787..4797275	-	489	WP_001559914.1	hypothetical protein	-
G9173_RS23725	4797272..4797649	-	378	WP_001559913.1	TA system toxin CbtA family protein	Toxin
G9173_RS23730	4797739..4798107	-	369	WP_032408847.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
G9173_RS23735	4798284..4798505	-	222	WP_000692300.1	DUF987 domain-containing protein	-
G9173_RS23740	4798574..4799050	-	477	WP_032408846.1	RadC family protein	-
G9173_RS23745	4799065..4799550	-	486	WP_088607457.1	antirestriction protein	-
G9173_RS23750	4799642..4800460	-	819	WP_001559911.1	DUF945 domain-containing protein	-
G9173_RS23755	4800560..4800793	-	234	WP_001119729.1	DUF905 family protein	-
G9173_RS23760	4800872..4801327	-	456	WP_000581504.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4792733..4812889	20156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14148.10 Da
• Isoelectric Point: 7.8276

>T151832 WP_001559913.1 NZ_CP049947:c4797649-4797272 [Klebsiella pneumoniae]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T151832 NZ_CP065768:c119894-119622 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13682.62 Da
• Isoelectric Point: 7.8398

>AT151832 WP_032408847.1 NZ_CP049947:c4798107-4797739 [Klebsiella pneumoniae]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVMLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT151832 NZ_CP065768:c120157-119894 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A5E2RWI0

Antitoxin

• AlphaFold DB: A0A5E3EVB5