Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4151139..4151758 Replicon chromosome
Accession NZ_CP049947
Organism Klebsiella pneumoniae strain 19KM267

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag G9173_RS20595 Protein ID WP_002892050.1
Coordinates 4151540..4151758 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag G9173_RS20590 Protein ID WP_002892066.1
Coordinates 4151139..4151513 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9173_RS20580 4146291..4147484 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G9173_RS20585 4147507..4150653 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
G9173_RS20590 4151139..4151513 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
G9173_RS20595 4151540..4151758 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
G9173_RS20600 4151917..4152483 + 567 WP_002892030.1 maltose O-acetyltransferase -
G9173_RS20605 4152455..4152595 - 141 WP_004147370.1 hypothetical protein -
G9173_RS20610 4152616..4153086 + 471 WP_002892026.1 YlaC family protein -
G9173_RS20615 4153061..4154512 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
G9173_RS20620 4154613..4155311 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
G9173_RS20625 4155308..4155448 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
G9173_RS20630 4155448..4155711 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T151830 WP_002892050.1 NZ_CP049947:4151540-4151758 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T151830 NZ_CP065767:182422-182688 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT151830 WP_002892066.1 NZ_CP049947:4151139-4151513 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT151830 NZ_CP065767:182159-182422 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References