Detailed information of TA system TA151801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4242081..4242706
• Replicon: chromosome
• Accession: NZ_CP049936
• Organism: Escherichia coli strain JL05

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G9V97_RS20135
• Protein ID: WP_000911330.1
• Coordinates: 4242308..4242706 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: G9V97_RS20130
• Protein ID: WP_000450524.1
• Coordinates: 4242081..4242308 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9V97_RS20105	4237884..4238354	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
G9V97_RS20110	4238354..4238926	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
G9V97_RS20115	4239072..4239950	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
G9V97_RS20120	4239967..4241001	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
G9V97_RS20125	4241214..4241927	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
G9V97_RS20130	4242081..4242308	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G9V97_RS20135	4242308..4242706	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
G9V97_RS20140	4242853..4243716	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
G9V97_RS20145	4243731..4245746	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
G9V97_RS20150	4245820..4246518	+	699	WP_000679823.1	esterase	-
G9V97_RS20155	4246628..4246828	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T151801 WP_000911330.1 NZ_CP049936:4242308-4242706 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T151801 NZ_CP065743:1137204-1137554 [Bacillus tropicus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT151801 WP_000450524.1 NZ_CP049936:4242081-4242308 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT151801 NZ_CP065743:1136912-1137199 [Bacillus tropicus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33