Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2412497..2412994 Replicon chromosome
Accession NZ_CP049936
Organism Escherichia coli strain JL05

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag G9V97_RS11410 Protein ID WP_001173343.1
Coordinates 2412497..2412670 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag G9V97_RS11415 Protein ID WP_000398619.1
Coordinates 2412698..2412994 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9V97_RS11400 2409167..2410624 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
G9V97_RS11405 2410861..2412378 + 1518 WP_001295074.1 lysine--tRNA ligase -
G9V97_RS11410 2412497..2412670 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
G9V97_RS11415 2412698..2412994 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
G9V97_RS11420 2413221..2413493 - 273 WP_000405647.1 N-acetyltransferase -
G9V97_RS11425 2413505..2413735 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
G9V97_RS11430 2413916..2415547 + 1632 WP_001216461.1 sensor histidine kinase -
G9V97_RS11435 2415544..2416263 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T151795 WP_001173343.1 NZ_CP049936:c2412670-2412497 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T151795 NZ_CP065729:559263-559535 [Macrococcus caseolyticus]
ATGCCTAAGAAAAAGATACTATTTTATTCAAGTGGTTTTGAAGATTATACGTACTGGCAATCTAACGATAAAACTGTATT
AAAGAGAATAAATAAACTAATAAAGAGTATTATGATTGATGGTGCAAATGAAGGTATAGGTAAGCCAGAAAAATTATTAG
GAAACTATACAGGTTGCTATAGCAGAAGGATCACTCATGAACATAGGCTCGTATATAAGATAGAAGATGATTCCATTTGT
ATTTTACAATGTCGTTTTCATTATAAAAGGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT151795 WP_000398619.1 NZ_CP049936:c2412994-2412698 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT151795 NZ_CP065729:559013-559270 [Macrococcus caseolyticus]
ATGAAAGTCACAACTTATTCTAATGCAAGATCAAATTTTAGAAAAATAATAGATATTGTTAATGAAGACTGTGAACCTTA
CACAATCACAACAAATACGTATAATGCCGTGATATTATCTGAACAAGATTATAATAGTATGATTGAAACACTTTATTTAC
AGAGTACACCAGCGAATGCCGAAAGATTACAAAAAGCGATTGATGAATCAATTGATAAAAATAACTTACTTCAAGTTGAG
ATTGATTTAGATGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References