Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 568330..568968 Replicon chromosome
Accession NZ_CP049936
Organism Escherichia coli strain JL05

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag G9V97_RS02715 Protein ID WP_000813794.1
Coordinates 568792..568968 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag G9V97_RS02710 Protein ID WP_001270286.1
Coordinates 568330..568746 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9V97_RS02690 563482..564423 - 942 WP_001251319.1 ABC transporter permease -
G9V97_RS02695 564424..565437 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
G9V97_RS02700 565455..566600 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
G9V97_RS02705 566845..568251 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
G9V97_RS02710 568330..568746 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
G9V97_RS02715 568792..568968 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
G9V97_RS02720 569190..569420 + 231 WP_000494244.1 YncJ family protein -
G9V97_RS02725 569512..571473 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
G9V97_RS02730 571546..572082 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
G9V97_RS02735 572135..573349 + 1215 WP_165746299.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 573389..574537 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T151788 WP_000813794.1 NZ_CP049936:c568968-568792 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T151788 NZ_CP065725:c307248-306973 [Oligella ureolytica]
ATGGCTTGGAAGATTGAGTTAACAAAAGAAGTAGCTAAAAAAATTAGAAAACTCGATAAACCAGTAGCTAAGCGACTTAT
TCAGTTTTTGCACGAACGTGTTTCGACCTTGAATAATCCAAGAGACATTGGGGAAGCCCTAACTGGTAGCAAACTAGGTG
AGTTTTGGAAATATCGTGTCGGTGACTACAGAATAATTTGTAAAATTGAGGATAAAGAAATAACTATTTTAGTTGTCCAG
ATAGGACATCGAAAGTCTATATATAACAACGATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT151788 WP_001270286.1 NZ_CP049936:c568746-568330 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT151788 NZ_CP065725:c307471-307232 [Oligella ureolytica]
ATGAATAAATCGACTAACGTATCTTTGCGTTTGCCAACAGAGCTTGTTGAGCGTATTAGTCACTTAGCTGAAACCACTGG
TCGAAGTCGCACATATTATATGCAAGAAGCTATCAAGCAACATATCGATGATTTAGAAGATCTTTATTTGGCTGAGCAAG
CATTGGTAGATGTGCGGGCAGGAAAAAGTGAAACTATTTCGTTAGAAACTTTAATTGAAGAATATGGCTTGGAAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References