Detailed information of TA system TA151777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1876613..1877412
• Replicon: chromosome
• Accession: NZ_CP049934
• Organism: Leucobacter insecticola strain HDW9B

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: G7067_RS08640
• Protein ID: WP_205881108.1
• Coordinates: 1876948..1877412 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: G7067_RS08635
• Protein ID: WP_166323527.1
• Coordinates: 1876613..1876909 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7067_RS08630	1875080..1876555	+	1476	WP_166323525.1	glutamate synthase subunit beta	-
G7067_RS08635	1876613..1876909	+	297	WP_166323527.1	DUF1778 domain-containing protein	Antitoxin
G7067_RS08640	1876948..1877412	+	465	WP_205881108.1	GNAT family N-acetyltransferase	Toxin
G7067_RS08645	1877494..1878138	+	645	WP_166323531.1	NAD(P)H-binding protein	-
G7067_RS08650	1878174..1879748	+	1575	WP_166323533.1	methyltransferase	-
G7067_RS08655	1879761..1881076	+	1316	Protein_1703	histidinol dehydrogenase	-
G7067_RS08660	1881076..1881573	+	498	WP_166323535.1	transcriptional regulator NrdR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (36-132)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 16279.68 Da
• Isoelectric Point: 9.8552

>T151777 WP_205881108.1 NZ_CP049934:1876948-1877412 [Leucobacter insecticola]
VAHFESGEPALDRYLVTRALPNHVAGFARCYVSVDSETGSVAGFYTLSAVAIEHAELPGRARRNAPNPVPAVLLGRLAVD
RAAQGAGLGRLLVRDALLSTLSAADRIGVRLLLVHALHGKAASFYSNFGFRPSPTDPLHLYVLLGDVRASLAKG

Nucleotide

• Length: 465 bp

>T151777 NZ_CP065712:738052-738414 [Staphylococcus auricularis]
ATGATGAGACGAGGAGATGTATATTTAGCTGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGGCCTGTTGT
TATCATTCAAAATGATACTGGGAATAAATATAGTCCTACCGTAATTGTCGCTGCAATTACAGGTAGGATTAATAAAGCTA
AAATTCCAACCCATGTAGAAATAGACAAGAAGAAATATAAATTAGATAAAGATTCAGTTATCTTGTTAGAACAAATTCGA
ACATTAGATAAAAAGAGGCTGAAAGAGAAATTAACATATCTATCTGACGAGAAGATGGAAGAAGTAAATCGTGCTATCTT
CATAAGTTTAGGGTTACATAATATGAAACATTTCAAATCATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10870.22 Da
• Isoelectric Point: 4.4734

>AT151777 WP_166323527.1 NZ_CP049934:1876613-1876909 [Leucobacter insecticola]
MSQAHETVQRKLARLAVRLTPDQDALIRDAAATSGQSISEFVTTAALTRAEDTLVDRRLFRLDDQAWAEFSAILDRPAQR
LPELAALLNDPAPWDEAV

Nucleotide

• Length: 297 bp

>AT151777 NZ_CP065712:737885-738055 [Staphylococcus auricularis]
ATGTCTAATTTTAACACTACGAGATATCACAATATGGAGCAAATCTTAAAAGAAGGCTATTCACAAATGGCCGATCTTAA
TCTCTCCCTAGCAACAGAAGCTTTTCCTTTCGAATGTGAAGCTTGTGATTGTAACGAATCATTCCTCAGCTCCAATCACA
ATAATGAATGA