151770

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1309180..1310096 Replicon chromosome
Accession NZ_CP049924
Organism Bacillus subtilis strain So1b

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag G4G26_RS06815 Protein ID WP_003244695.1
Coordinates 1309350..1310096 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag G4G26_RS06810 Protein ID WP_003232646.1
Coordinates 1309180..1309350 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4G26_RS06775 1306043..1306372 + 330 WP_182932248.1 XkdW family protein -
G4G26_RS06780 1306369..1306533 + 165 WP_041850927.1 XkdX family protein -
G4G26_RS06785 1306577..1307416 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
G4G26_RS06790 1307469..1307738 + 270 WP_003232655.1 hemolysin XhlA family protein -
G4G26_RS06795 1307751..1308014 + 264 WP_003232653.1 phage holin -
G4G26_RS06800 1308027..1308920 + 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
G4G26_RS06805 1308957..1309094 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
G4G26_RS06810 1309180..1309350 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
G4G26_RS06815 1309350..1310096 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
G4G26_RS06820 1310206..1311207 - 1002 WP_003232642.1 inorganic phosphate transporter -
G4G26_RS06825 1311220..1311837 - 618 WP_003218470.1 DUF47 domain-containing protein -
G4G26_RS06830 1312113..1313429 - 1317 WP_041850924.1 serine/threonine exchanger -
G4G26_RS06835 1313817..1314767 + 951 WP_041344800.1 ring-cleaving dioxygenase -
G4G26_RS06840 1314868..1315014 + 147 WP_120363335.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1274040..1320052 46012


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T151770 WP_003244695.1 NZ_CP049924:c1310096-1309350 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T151770 NZ_CP065710:c1776910-1776803 [Staphylococcus equorum]
GTGGTTCCAATTGTAGAAGCACTACACTTAATGTTAGGTTTCGGTACTTTCATCGTTACCTTATTAGGTTTAGTGGTTGC
AATTGTTAAATTAAGCCATAAAAAATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT151770 WP_003232646.1 NZ_CP049924:c1309350-1309180 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT151770 NZ_CP065710:1776690-1776746 [Staphylococcus equorum]
AAAAAGGGCAACACACCATAAGTGTATCGCCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References