Detailed information of TA system TA151769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 521406..522042
• Replicon: chromosome
• Accession: NZ_CP049924
• Organism: Bacillus subtilis strain So1b

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: G4G26_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 521692..522042 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: G4G26_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 521406..521687 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4G26_RS02600	517765..518364	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
G4G26_RS02605	518459..518824	+	366	WP_003234281.1	holo-ACP synthase	-
G4G26_RS02610	518990..520006	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
G4G26_RS02615	520121..521290	+	1170	WP_003234284.1	alanine racemase	-
G4G26_RS02620	521406..521687	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
G4G26_RS02625	521692..522042	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
G4G26_RS02630	522157..522981	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
G4G26_RS02635	522986..523351	+	366	WP_015715277.1	RsbT antagonist protein RsbS	-
G4G26_RS02640	523355..523756	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
G4G26_RS02645	523768..524775	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
G4G26_RS02650	524837..525166	+	330	WP_014475877.1	anti-sigma factor antagonist RsbV	-
G4G26_RS02655	525163..525645	+	483	WP_038427419.1	anti-sigma B factor RsbW	-
G4G26_RS02660	525611..526399	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
G4G26_RS02665	526399..526998	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T151769 WP_003156187.1 NZ_CP049924:521692-522042 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T151769 NZ_CP065707:890667-891032 [Streptococcus oralis]
GTGCATACGATTTACTTCTATAAGGACAAAAATGGAAATGAGCCAGTCTTAGATTATATGAGACAATTGGCTAGAAAGAA
GAGTAAGGACAGTCGCATCAAACTCAATAAACTAAACGACTATATCGAGTTGCTTAGTCAGCATGGGACTAGAACTGGTG
AACCCTATATCAAACATTTGGAAGATGAGATTTGGGAACTACGACCTCTTAAGGATAGGATTTTATTTGTAGCATGGGTT
GATGGGAGTTTTGTTCTTTTACATCATTTTGTTAAAAAGACTCAGAAAACTCCTAGGAGAGAGATTGATAAATCCAAGCG
TGAACTGAAGGACATAAAAGAAAGAGGGTTAAGTGATGAAGAATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT151769 WP_003225183.1 NZ_CP049924:521406-521687 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT151769 NZ_CP065707:891022-891315 [Streptococcus oralis]
ATGAAGAATAGTGCCATTGGAAGTAATTGGAAGGATATCCGAACAGAACTCTTTACCAAGGAAGAAATTCTTGAAAGTGA
TATGCGAGTGGCTATTATGAGTGAGTTGATTGAGGCTAGACATGAGCAAGGTATCAGTCAGAAAAAGCTAGAGGAACTCA
GTGGAGTAAGCCAGCCTGTCATAGCTAGGATGGAAACAGGAAAGACTAGTCCTCAGTTGGATACAGTCTTGAAAGTTCTA
GCCAGTTTAGGAAAGACACTAGCAGTCGTCCCACTAGAACAGGAAAAAAATTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32