Detailed information of TA system TA151750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1230858..1231775
• Replicon: chromosome
• Accession: NZ_CP049904
• Organism: Bacillus velezensis strain GB03

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: V753_RS06415
• Protein ID: WP_007407256.1
• Coordinates: 1231029..1231775 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: V753_RS06410
• Protein ID: WP_003154807.1
• Coordinates: 1230858..1231028 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
V753_RS06370 (1226082)	1226082..1227713	+	1632	WP_007610826.1	hypothetical protein	-
V753_RS06375 (1227726)	1227726..1228097	+	372	WP_007610831.1	XkdW family protein	-
V753_RS06380 (1228102)	1228102..1228299	+	198	WP_007610833.1	XkdX family protein	-
V753_RS06385 (1228356)	1228356..1229117	+	762	WP_029325867.1	hypothetical protein	-
V753_RS06390 (1229169)	1229169..1229432	+	264	WP_003154815.1	hemolysin XhlA family protein	-
V753_RS06395 (1229446)	1229446..1229709	+	264	WP_003154813.1	phage holin	-
V753_RS06400 (1229723)	1229723..1230601	+	879	WP_007610836.1	N-acetylmuramoyl-L-alanine amidase	-
V753_RS06405 (1230636)	1230636..1230761	-	126	WP_003154809.1	hypothetical protein	-
V753_RS06410 (1230858)	1230858..1231028	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
V753_RS06415 (1231029)	1231029..1231775	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
V753_RS06420 (1231880)	1231880..1232878	-	999	WP_007610840.1	inorganic phosphate transporter	-
V753_RS06425 (1232891)	1232891..1233508	-	618	WP_003154804.1	DUF47 domain-containing protein	-
V753_RS06430 (1233794)	1233794..1235110	-	1317	WP_007610842.1	amino acid permease	-
V753_RS06435 (1235433)	1235433..1236383	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T151750 WP_007407256.1 NZ_CP049904:c1231775-1231029 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T151750 NZ_CP065695:6009443-6009853 [Delftia acidovorans]
ATGATGCTGTACATGCTGGACACCAATGCCGCCAGCGAAGCCATCCGGGGCAATCCCTTGTTTGATGCGCGCTTGCAAGC
CCTCGCACCAGGGCAGTGGTGCGTTAGTGCAGTGACCTGTTCCGAGCTGCGCTATGGAATTGCCCGGCGGCCCGAGGCGG
TACGGCTGGCACGGATCGTGGAGGCGTTCCTGCACATCACCGCCATCCTGCCCTGGGACGCCAGGGCGGCCAACCAGCAT
GGTCAGTTGCGTGCTTATCTGCGTGCCCAGGGCTCGCCGATTGGAGACTTCGACGAAATGATCGCCGCACATGCGCTGGC
CCTGGACGCGATCCTGGTCACAGACAATGTGCGGCATTTCGAACGCATTTCCGGGCTCAGGATCGAAAACTGGCTGCGCC
AGGGGCATTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT151750 WP_003154807.1 NZ_CP049904:c1231028-1230858 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT151750 NZ_CP065695:6009180-6009446 [Delftia acidovorans]
ATGCCACCCGTTACCGCCCAACTTTTCGCCACCGATGGCGTTCAAGCCGTAAAGCTGCCTGCCGAGTTTCGCTTCGAAGA
TCGAGCCGAGGTGTATATCCGCCGCGATGAAAACACCGGAGACGTCATTCTTTCGACCATTCCTCGCGCGGACTGGAGTG
AATTCATGCGGCTGCGCCACCAGCTGGGGCATGCGGCCGAGTCGGCCCTCCCCGTGCGCCAACAGGGCAGCGAGCAACGC
GATCCCTTCCAAGGTTGGGAGGAATGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14