Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 95425..96068 Replicon plasmid pEcoUTI1d
Accession NZ_CP049853
Organism Escherichia coli strain UPEC-774U/190308

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag G9W34_RS24745 Protein ID WP_001034044.1
Coordinates 95652..96068 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag G9W34_RS24740 Protein ID WP_001261286.1
Coordinates 95425..95655 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W34_RS24725 (G9W34_24660) 90562..90792 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
G9W34_RS24730 (G9W34_24665) 90789..91205 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
G9W34_RS24735 (G9W34_24670) 91250..95044 - 3795 WP_001144732.1 hypothetical protein -
G9W34_RS24740 (G9W34_24675) 95425..95655 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
G9W34_RS24745 (G9W34_24680) 95652..96068 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
G9W34_RS24750 (G9W34_24685) 96143..97708 + 1566 WP_001128474.1 AAA family ATPase -
G9W34_RS24755 (G9W34_24690) 97693..98715 + 1023 WP_000361402.1 helicase UvrD -
G9W34_RS24765 (G9W34_24700) 99969..100666 + 698 WP_094096600.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id senB 1..102591 102591


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T151735 WP_001034044.1 NZ_CP049853:95652-96068 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T151735 NZ_CP065693:c369161-368877 [Enterobacter asburiae]
ATGACTTATGAACTGGCATTTGATCCGAGGGCGTTAAAGGAGTGGAGCAAGCTGGGGGAGACTGTAAAAGACCAGTTTAA
GAAGAAACTTGCGGAGGTGTTGGTGAATCCCAGGGTCGAATCGGCCCGCCTGCATGGTCTACCGGATTGCTACAAAATAA
AACTCAGATCGCAGGGTTATCGTTTGGTCTATCAGGTTCAGGACAACGTTGTAACGGTATTTGTTATCGCTATCGGTAAG
AGAGAGAAATCAGCCGTTTATCATGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT151735 WP_001261286.1 NZ_CP049853:95425-95655 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT151735 NZ_CP065693:c369393-369151 [Enterobacter asburiae]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACGGAAGCCGTGCGATTACTGTTTCAGTACGTCGCGGAGACGGGACGTATGCCGGTGAAAACGGTCACGATTAGCGACA
GTGAAGATGCGCTGATACAGACGGTCCGGGAGCGACTGGCGAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References