Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3807155..3807809 Replicon chromosome
Accession NZ_CP049852
Organism Escherichia coli strain UPEC-774U/190308

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag G9W34_RS18475 Protein ID WP_000244781.1
Coordinates 3807155..3807562 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G9W34_RS18480 Protein ID WP_000354046.1
Coordinates 3807543..3807809 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W34_RS18455 (3803112) 3803112..3804845 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
G9W34_RS18460 (3804851) 3804851..3805561 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G9W34_RS18465 (3805586) 3805586..3806482 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G9W34_RS18470 (3806594) 3806594..3807115 + 522 WP_001055874.1 flavodoxin FldB -
G9W34_RS18475 (3807155) 3807155..3807562 - 408 WP_000244781.1 protein YgfX Toxin
G9W34_RS18480 (3807543) 3807543..3807809 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G9W34_RS18485 (3808052) 3808052..3809032 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
G9W34_RS18490 (3809109) 3809109..3809768 - 660 WP_000250274.1 hemolysin III family protein -
G9W34_RS18495 (3809932) 3809932..3810243 - 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
G9W34_RS18500 (3810288) 3810288..3811721 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T151730 WP_000244781.1 NZ_CP049852:c3807562-3807155 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T151730 NZ_CP065688:3189630-3189899 [Serratia plymuthica]
ATGCGGATAGAATGGAGTCGTGAAGCTCAGGAAGAGCTATGGAACATTATTGATTATATTGATGATAGAAACCCGCAGGC
GGCGCTCATTTTACAGCGCGAGATCGAAACGGCGGTGCATTCGCTGTCGCTGAATCCCTTTATGTACCGGTTGGGCAGAG
TAGATAACACTCGTGAGATTGTCGTCCATCCCAACTACCTGATTGTTTATAGAATCATCGGACATATAGAAGTTCTTGGC
GTGATTCACGCTAAACGAGAATATCCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT151730 WP_000354046.1 NZ_CP049852:c3807809-3807543 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT151730 NZ_CP065688:3189432-3189626 [Serratia plymuthica]
ATGGTGATCAAAACCTCCCTCTCCACCCAGCCCACAGCTGAGGTAGCCGAACAAGGCGAAAGTTACGATCGTTGGTTCCG
GGCCAAGATTGAAGCAGCCATTAACAGCGAAAAGCCAAGAATTCCGCACGAGCAGGTCATGAACGAGATGCAAATTCGTT
TAGCGGCGAAGAAAAAGATACGCAAAATGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References