Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1203955..1204573 Replicon chromosome
Accession NZ_CP049852
Organism Escherichia coli strain UPEC-774U/190308

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G9W34_RS05735 Protein ID WP_001291435.1
Coordinates 1203955..1204173 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G9W34_RS05740 Protein ID WP_000344800.1
Coordinates 1204199..1204573 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W34_RS05700 (1199243) 1199243..1199815 + 573 WP_000779831.1 YbaY family lipoprotein -
G9W34_RS05705 (1199846) 1199846..1200157 - 312 WP_000409908.1 MGMT family protein -
G9W34_RS05715 (1200536) 1200536..1200889 + 354 WP_000878140.1 DUF1428 domain-containing protein -
G9W34_RS05720 (1200931) 1200931..1202481 - 1551 WP_001538392.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G9W34_RS05725 (1202645) 1202645..1203115 - 471 WP_000136192.1 YlaC family protein -
G9W34_RS05730 (1203231) 1203231..1203782 - 552 WP_000102539.1 maltose O-acetyltransferase -
G9W34_RS05735 (1203955) 1203955..1204173 - 219 WP_001291435.1 HHA domain-containing protein Toxin
G9W34_RS05740 (1204199) 1204199..1204573 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G9W34_RS05745 (1205119) 1205119..1208268 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
G9W34_RS05750 (1208291) 1208291..1209484 - 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T151717 WP_001291435.1 NZ_CP049852:c1204173-1203955 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T151717 NZ_CP065686:c3990504-3990172 [Burkholderia humptydooensis]
ATGACGCAAAATTGGACTATTGTCTACTATAACGAGCGCGTCAAGCGCGACGTTTTCGCACTGCCTGCGGGGATCTTGGC
GGATTATCTGCGGCTCCTAGATTTGATGCAGGAGTTTGGTGCTGATCTGCGCATGCCGCATTCGCGGGCAATGGGGGGCG
GATTGTTTGAGTTGCGCCCAAAAGGCAGGGAAGGCATCGGGCGCGTGTTTTACTGCACCCATGTTGGACAGCGGGTCGTG
GTCCTGCATTCGTTTGTGAAGAAAACGCAGGAGACGCCTCAGAATGAGCTGCGAACCGCTCGGGTGCGTTTAAGTGAGGT
GCGTAATGGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT151717 WP_000344800.1 NZ_CP049852:c1204573-1204199 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT151717 NZ_CP065686:c3990164-3989847 [Burkholderia humptydooensis]
GTGAAGCGTGCTAGTGCTGAAGGCTTCAACCCGATCCAGCACACGGTAGATGATACGGAGCATCTGCTGGCAAAGCCGGG
AGTCAGAGTGGCGTATGATGCTCTGGAAGATGAGTACATGGCTCTGCGGGCCATTTTGGCTATTCGGCATGAGGCCGGAT
TGACGCAAGCTCAAGTGGCAGAGCGCATGGGAACTACGGCGTCAGCGGTTTCGCGACTTGAGGCATCTCTGTCTAGTGAA
AAGCACTCACCCTCCTTTGCGACGCTGCGCAAGTATGCTGCTGCGTGTGGCAAGAGATTGGTGATTTCGTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References