Detailed information of TA system TA151715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 729316..729866
• Replicon: chromosome
• Accession: NZ_CP049852
• Organism: Escherichia coli strain UPEC-774U/190308

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PDG4
• Locus tag: G9W34_RS03505
• Protein ID: WP_001160966.1
• Coordinates: 729552..729866 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1NMQ0
• Locus tag: G9W34_RS03500
• Protein ID: WP_000125568.1
• Coordinates: 729316..729549 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W34_RS03475 (724389)	724389..724676	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
G9W34_RS03480 (724735)	724735..726066	+	1332	WP_001183207.1	MFS transporter	-
G9W34_RS03485 (726174)	726174..726704	+	531	WP_000600732.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
G9W34_RS03490 (726697)	726697..728559	+	1863	WP_000377109.1	glutathione-regulated potassium-efflux system protein KefC	-
G9W34_RS03495 (728751)	728751..729230	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
G9W34_RS03500 (729316)	729316..729549	+	234	WP_000125568.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G9W34_RS03505 (729552)	729552..729866	+	315	WP_001160966.1	CcdB family protein	Toxin
G9W34_RS03510 (729863)	729863..730711	-	849	WP_000257181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
G9W34_RS03515 (730718)	730718..731095	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
G9W34_RS03520 (731098)	731098..731919	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
G9W34_RS03525 (731916)	731916..732905	-	990	WP_000241225.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
G9W34_RS03530 (732905)	732905..734191	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.72 Da
• Isoelectric Point: 9.0851

>T151715 WP_001160966.1 NZ_CP049852:729552-729866 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T151715 NZ_CP065685:c54968-54558 [Burkholderia humptydooensis]
ATGACGCTGTATCTGCTCGACACCAACATTTTGTCGAACGTGATCCGAGACCCGCGCGGCGCATGCACCGCCCGCATCGG
AGAAACACCGCCCGAACAAGTGTGCACCTCGATCATCGTGGCCGCGGAACTCCGGTTTGGCGTCTGGAAGCGCGGATCGT
CGACGCTCGCGCAGCGCGTCGAGCAACTGCTCGCGAGCTTGACCGTGTTGCCGCTGCAGCCTGACGCAGACCGTTGCTAC
GGCCGGTTGCGGGCCGAGCTCGAGAAGCAAGGCCAGTTGATCGGCGCCAACGACATGCTAATCGCCGCGCATGCGCTGGC
CGTGGACGCCGTCCTGGTCACCGACAACACGGCGGAATTTGCTCGGGTCGCGGGGCTGCCAGTCGAAAACTGGTTGCGCC
CGGCGACGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.55 Da
• Isoelectric Point: 5.8062

>AT151715 WP_000125568.1 NZ_CP049852:729316-729549 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT151715 NZ_CP065685:c55207-54965 [Burkholderia humptydooensis]
ATGCCAACCCATGACACCCGCCACGCGCGCCTGTTTCGCAATGGGCGCAGCCAGGCCGTGCGGATCCCGCGCGAGTTCGA
GCTACCGGTCTCGGAAGTCACTATCCACCGGGAAGGCCGGCGCTTGATTATCGAGCCCGTGGAGGCCGCGCCCCTGCGTG
AGCTGTTCGCCCAATGGACGCCGCTGGACGAAACCTTTCCCGACATCGATAACCTGCCGGCTGAACCGGTCGACATCGAA
TGA

Structures

Toxin

• AlphaFold DB: A0A829G815

Antitoxin

• AlphaFold DB: A0A829GEF2