Detailed information of TA system TA151700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 58201..58802
• Replicon: plasmid pEcoUTI1e
• Accession: NZ_CP049847
• Organism: Escherichia coli strain UPEC-245U/190328

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: G9W35_RS25345
• Protein ID: WP_001216034.1
• Coordinates: 58201..58581 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A403D1Y2
• Locus tag: G9W35_RS25350
• Protein ID: WP_001190714.1
• Coordinates: 58581..58802 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W35_RS25320 (G9W35_25215)	53641..55083	-	1443	WP_001472843.1	hypothetical protein	-
G9W35_RS25325 (G9W35_25220)	55125..56318	-	1194	WP_000219608.1	hypothetical protein	-
G9W35_RS25330 (G9W35_25225)	56405..56857	-	453	WP_001312282.1	late promoter-activating protein (Gp10)	-
G9W35_RS25335 (G9W35_25230)	56946..57989	-	1044	WP_221524496.1	DUF968 domain-containing protein	-
G9W35_RS25340 (G9W35_25235)	58017..58196	-	180	WP_000113019.1	hypothetical protein	-
G9W35_RS25345 (G9W35_25240)	58201..58581	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
G9W35_RS25350 (G9W35_25245)	58581..58802	-	222	WP_001190714.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
G9W35_RS25355 (G9W35_25250)	58875..59264	-	390	WP_000506730.1	S24 family peptidase	-
G9W35_RS25360 (G9W35_25255)	59439..60023	+	585	WP_053893011.1	hypothetical protein	-
G9W35_RS25365 (G9W35_25260)	60024..60380	+	357	WP_001062545.1	hypothetical protein	-
G9W35_RS25370 (G9W35_25265)	61456..61818	-	363	WP_001261543.1	hypothetical protein	-
G9W35_RS25375 (G9W35_25270)	61815..62489	-	675	WP_032236592.1	hypothetical protein	-
G9W35_RS25380 (G9W35_25275)	62471..62845	-	375	WP_000988652.1	hypothetical protein	-
G9W35_RS25385 (G9W35_25280)	62852..63145	-	294	WP_221524497.1	hypothetical protein	-
G9W35_RS25390 (G9W35_25285)	63324..63557	-	234	WP_000517421.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90408	90408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T151700 WP_001216034.1 NZ_CP049847:c58581-58201 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T151700 NZ_CP065673:1700175-1700465 [Serratia plymuthica]
GTGCCACAACTGATATGGACGCCAGCGGCGCTGCTGGATGTACAGCAGCTTTATCGATTTCTGGCTGAGAAGGATCTCAA
TTCGGCAAAGCGCGCCGTCGCCACTATTCGTAACTGCATCAAAATAATAGCGCACCAACCCCATATTGGCCGCCCCGCTA
AAGATATGGCACCTGAATTCAGGGAGTGGCCTATCGATTTCGGCAATAGCGGTTATCTTGTTCTGTATCGGTTTAACGGT
GTAACCGCCGTTATTTTGGCTATTCGCCACCAAAGCGAAACCGGGTATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8123.06 Da
• Isoelectric Point: 4.7708

>AT151700 WP_001190714.1 NZ_CP049847:c58802-58581 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGRESAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT151700 NZ_CP065673:1699909-1700187 [Serratia plymuthica]
ATGGCTACTGCTACGTCGATAAAAATTGATGATGAGCTTAAACAACGGGTGCAACACATTGCCGCGGCCCATCAGCGCAG
CGCACACTGGATTATGCGTGAAGCCATTCGTGAATATGTCGAGCGTGAAGAAAAACGCGAGGCTGTTCGCAACGATGCAT
TACAGGCATGGGAAACCTATCAGGCCAATGGTAAACATTTGACTCAGCACGAGGCTGACGGCTGGCTCTCCGATCTGGAA
AAAGGTGATGATACGGAGATACCCGGGTGCCACAACTGA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A403D1Y2