Detailed information of TA system TA151695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3638011..3638649
• Replicon: chromosome
• Accession: NZ_CP049845
• Organism: Escherichia coli strain UPEC-245U/190328

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: G9W35_RS17610
• Protein ID: WP_000813794.1
• Coordinates: 3638473..3638649 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G9W35_RS17605
• Protein ID: WP_001270286.1
• Coordinates: 3638011..3638427 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W35_RS17585 (3633163)	3633163..3634104	-	942	WP_001251319.1	ABC transporter permease	-
G9W35_RS17590 (3634105)	3634105..3635118	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
G9W35_RS17595 (3635136)	3635136..3636281	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
G9W35_RS17600 (3636523)	3636523..3637932	-	1410	WP_000760591.1	PLP-dependent aminotransferase family protein	-
G9W35_RS17605 (3638011)	3638011..3638427	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
G9W35_RS17610 (3638473)	3638473..3638649	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
G9W35_RS17615 (3638871)	3638871..3639101	+	231	WP_000494244.1	YncJ family protein	-
G9W35_RS17620 (3639193)	3639193..3641154	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
G9W35_RS17625 (3641227)	3641227..3641763	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
G9W35_RS17630 (3641855)	3641855..3643030	+	1176	WP_021565101.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3643070..3644335	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T151695 WP_000813794.1 NZ_CP049845:c3638649-3638473 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T151695 NZ_CP065669:c48860-48447 [Sphingomonas paucimobilis]
GTGACCCAGCTCTACATGCTCGACACCAACATCGTGTCGGAACTCGCGCGAAACCCGCACGGCGCCGTAGCAACCCGCAT
CGCGCAAGTCGGTGCCGATGCGATTTGCGTCAGCATCATCACCGCGGCGGAGCTGCGCTACGGCTGCGCGAAGAAAGGGT
CCCCCAGGCTCCTGGCCCAGATAGAGGCGATCCTCGGCAGCGTGTCCGTGCTTGCGCTCGATGTCCCGGCGGACACTGAA
TATGGCGGTATCCGAGCCGAGCTGGAGAGCGCGGGCAAACCCATCGGGCCCAACGACCTCTTCATCGCGGCTCATGCCTA
TGCGCTCGGCGCCACATTGGTGACCGCCAACATCGGCGAGTTCTCCCGCGTCCGGGCCCTCAAGGTGGAGAATTGGCTGA
GCGACGTCCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT151695 WP_001270286.1 NZ_CP049845:c3638427-3638011 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT151695 NZ_CP065669:c49120-48857 [Sphingomonas paucimobilis]
GTGACGCCCGATCATGAAGCAACGGTCACCAGAGAAGCGAAGCTGTTCCGCAACAACAAGAGCCAGGCAGTGCGCATCCC
GGCCGATTTCGAGCTGCCTGGGGATCGGGTCATGATCCACCGGGAGGGAGATCGACTGGTCATCGAGCCGATCCGGCGCC
GAAACATCGTCGAGGTCCTTGCGAGCCTCGAACCCCTTGGCCCCGAGGATGAGTTCCCGGACATCGACGGCACGTTGCTT
CCCGCCAAGGCCGTCGAATTGTGA

Structures

Toxin

• PDB: 6HPB