Detailed information of TA system TA151683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2438884..2439509
• Replicon: chromosome
• Accession: NZ_CP049845
• Organism: Escherichia coli strain UPEC-245U/190328

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G9W35_RS11740
• Protein ID: WP_000911330.1
• Coordinates: 2439111..2439509 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: G9W35_RS11735
• Protein ID: WP_000450524.1
• Coordinates: 2438884..2439111 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G9W35_RS11710 (2434687)	2434687..2435157	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
G9W35_RS11715 (2435157)	2435157..2435729	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
G9W35_RS11720 (2435875)	2435875..2436753	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
G9W35_RS11725 (2436770)	2436770..2437804	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
G9W35_RS11730 (2438017)	2438017..2438730	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
G9W35_RS11735 (2438884)	2438884..2439111	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
G9W35_RS11740 (2439111)	2439111..2439509	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
G9W35_RS11745 (2439656)	2439656..2440519	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
G9W35_RS11750 (2440534)	2440534..2442549	+	2016	WP_074500075.1	tRNA cytosine(34) acetyltransferase TmcA	-
G9W35_RS11755 (2442623)	2442623..2443321	+	699	WP_000679812.1	esterase	-
G9W35_RS11760 (2443441)	2443441..2444421	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2443441..2444421	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T151683 WP_000911330.1 NZ_CP049845:2439111-2439509 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T151683 NZ_CP065663:c3189-2902 [Acinetobacter johnsonii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCACGATGTCTCTTTCGAAACAGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCGATGGTGAAGAACGTTGGCAAACCATCGGAA
AAGTAAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGGCAAGTCACCAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT151683 WP_000450524.1 NZ_CP049845:2438884-2439111 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT151683 NZ_CP065663:c2915-2601 [Acinetobacter johnsonii]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTGAATGATAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACTGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAACAATGCTGTACGCC
ATAATCAGTTCTACCGTCCAGTGAAGCAACAGACTTCCATTCGTTTAGATGCTGATGTGCTTGCTTGGTTCAAAGCACAA
GGGAAAGGTTATCAAACACGTATGAATGAAATCTTACGGGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33