Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2129899..2130482 Replicon chromosome
Accession NZ_CP049845
Organism Escherichia coli strain UPEC-245U/190328

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag G9W35_RS10250 Protein ID WP_000254745.1
Coordinates 2130147..2130482 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag G9W35_RS10245 Protein ID WP_000581937.1
Coordinates 2129899..2130147 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W35_RS10235 (2126238) 2126238..2127539 + 1302 WP_074500527.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
G9W35_RS10240 (2127587) 2127587..2129821 + 2235 WP_000226815.1 GTP pyrophosphokinase -
G9W35_RS10245 (2129899) 2129899..2130147 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
G9W35_RS10250 (2130147) 2130147..2130482 + 336 WP_000254745.1 endoribonuclease MazF Toxin
G9W35_RS10255 (2130553) 2130553..2131344 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
G9W35_RS10260 (2131572) 2131572..2133209 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
G9W35_RS10265 (2133297) 2133297..2134595 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T151682 WP_000254745.1 NZ_CP049845:2130147-2130482 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T151682 NZ_CP065662:c1515263-1514991 [Aerococcus urinae]
ATGAATAAAATTAGATTTACTAGTGCTTTCAAGCGGGATTATAAACGTTACAAGAAAAAGCACTATCCCATGACTAAAAT
TGATAAGGCTATATGCTTGATAGCTAGTGAAGAAATAGACATCTTAATTAATCAGTATAGGCTTCATAATCTAAAGGGAG
AATGGCAAGGATATAAAGAACTTCATGTTGATGGGGATACGCTAATTATTTATATTGATCAAAAAGGGATAGTAACCTTA
ACTAGGTTAGGAACACATGACCAACTTTTTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT151682 WP_000581937.1 NZ_CP049845:2129899-2130147 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT151682 NZ_CP065662:c1515507-1515256 [Aerococcus urinae]
ATGGCTAGAATCAATATTAGAGTTGATGATGATTTAAAAGAGCAAGCTAAAGCAACTTTTGAAGATATGGGGCTTGATCT
GACAACAGCAATCACTTTATTTATTAAGTATTCCGTGAATAGTGGTGAATTACCATTCAAACCAGCAACAAAGAAGCAAA
TTGAGAACAGACAAGCAAGGGCCGAAGCCGAAAGTGACCAACTAAAAACTTATGATAATGTCGATGATTTACTAGAGGCA
TTAAATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References