Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1993565..1994219 Replicon chromosome
Accession NZ_CP049845
Organism Escherichia coli strain UPEC-245U/190328

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag G9W35_RS09635 Protein ID WP_000244772.1
Coordinates 1993812..1994219 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G9W35_RS09630 Protein ID WP_000354046.1
Coordinates 1993565..1993831 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W35_RS09605 (1988853) 1988853..1989596 + 744 WP_021564907.1 SDR family oxidoreductase -
G9W35_RS09610 (1989653) 1989653..1991086 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
G9W35_RS09615 (1991131) 1991131..1991442 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
G9W35_RS09620 (1991606) 1991606..1992265 + 660 WP_000250274.1 hemolysin III family protein -
G9W35_RS09625 (1992342) 1992342..1993322 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
G9W35_RS09630 (1993565) 1993565..1993831 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G9W35_RS09635 (1993812) 1993812..1994219 + 408 WP_000244772.1 protein YgfX Toxin
G9W35_RS09640 (1994259) 1994259..1994780 - 522 WP_001055874.1 flavodoxin FldB -
G9W35_RS09645 (1994892) 1994892..1995788 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G9W35_RS09650 (1995813) 1995813..1996523 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G9W35_RS09655 (1996529) 1996529..1998262 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T151681 WP_000244772.1 NZ_CP049845:1993812-1994219 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T151681 NZ_CP065662:c1030094-1029762 [Aerococcus urinae]
ATGGTAAAAGTGAAGCAAGGATCGATTATCAAAATAAATTTAGATCCAAAACAGGGACATGAGCAAAAAGGCTATAGGCC
CTATATTTGTTTGAGCCATGGTATTGTTACTAAGTACTCAAATATTGCCATTTTTGCACCGATTAGCAATACAAAAAGAA
AATATCCCTTTTATGTAGAGTTAGAACAGACAAAAACAACTGGAAAAGTACTCTTAGATCAGTTAGTTACCATCGATTTT
AATGCCAGAGACTATAATTATATCGAACAAGTCACAGATGATATCCTCATTGATTTATTAGCTAGGGTAAAAGTATTATT
CGAAAAAGAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT151681 WP_000354046.1 NZ_CP049845:1993565-1993831 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT151681 NZ_CP065662:c1030333-1030088 [Aerococcus urinae]
ATGCCTTTAACAATTAATAAATGGGGGAATAGTTCTGCTATTCGATTACCAAAACAGTTGCTTGAAGAATTAAATTTATC
CCAAGGCGACAAACTGGATTATCAAGTGAAAGATGGTCAACTGATTATAAAAAAAATAACCGAAGTTCCTGAACTTACGG
TTAAAGATTTGTTTGAAAATTATGATGGAGCTCCTGTTAATGAAAAGCCCGTATTATTTGAACAAAGAGGAAATGAAAAA
TGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References