Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 292200..292776 Replicon chromosome
Accession NZ_CP049845
Organism Escherichia coli strain UPEC-245U/190328

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag G9W35_RS01430 Protein ID WP_001297643.1
Coordinates 292489..292776 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag G9W35_RS01425 Protein ID WP_000063160.1
Coordinates 292200..292502 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W35_RS01410 (288796) 288796..290946 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
G9W35_RS01415 (290996) 290996..291199 + 204 WP_000467859.1 YbdD/YjiX family protein -
G9W35_RS01420 (291210) 291210..292166 + 957 WP_001297640.1 GTPase -
G9W35_RS01425 (292200) 292200..292502 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
G9W35_RS01430 (292489) 292489..292776 - 288 WP_001297643.1 BrnT family toxin Toxin
G9W35_RS26130 (292975) 292975..293671 - 697 Protein_280 IS1 family transposase -
G9W35_RS01445 (293986) 293986..295218 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
G9W35_RS01450 (295259) 295259..296539 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 292975..293184 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T151674 WP_001297643.1 NZ_CP049845:c292776-292489 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T151674 NZ_CP065652:3561499-3561774 [Achromobacter xylosoxidans]
ATGGAAATGAAGTGGACCAGCAAGGCGCTTTCCGATCTGGCGCGCTTGCATGAATTCCTGGCGGCGGTGAATAGGCCCGC
GGCCGTACGGGCTGTGCAAGCGCTGGTCAAGGCTTCAAGTGTTTTGCCGACCAACTCCCGCATTGGCGAGCAGCTATTTC
AGTTCGAACCGCGCGAGGTACGCAGGATATTGGTCGGGGAGTACGAAATACGTTACGAGATCCGGGATTCGATCATCTAC
GTATTGCGACTGTGGCACACCCGGGAGAACCGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT151674 WP_000063160.1 NZ_CP049845:c292502-292200 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT151674 NZ_CP065652:3561233-3561499 [Achromobacter xylosoxidans]
ATGAGTCCCGCAACCAAGGTGCTGACCGCACATGTGCCGCTTCCGTTGGCCGAGAAGGTCGATCAGATGGCTGTGCGCCT
GGACCGATCTCGCGGCTGGATCGTGAAGCAGGCACTGTCCGCTTGGATTGCCCAAGAGGAGGAACGTGACAAGCTCACCG
AGCAAGCGCTTGCTGACGTGGATGCTGGTCGGATTGTGGATGACCAAGCCGTGCAAGCCTGGGCGGAGAGCCTGGGCTCT
GACCAGCCATTGCCGCTGCCGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References