Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 728372..728922 Replicon chromosome
Accession NZ_CP049839
Organism Escherichia coli strain UPEC-455U/190220

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag G9W36_RS03500 Protein ID WP_001160966.1
Coordinates 728608..728922 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag G9W36_RS03495 Protein ID WP_000125568.1
Coordinates 728372..728605 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G9W36_RS03470 (723445) 723445..723732 + 288 WP_000203741.1 ferredoxin-like protein FixX -
G9W36_RS03475 (723791) 723791..725122 + 1332 WP_001183207.1 MFS transporter -
G9W36_RS03480 (725230) 725230..725760 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
G9W36_RS03485 (725753) 725753..727615 + 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
G9W36_RS03490 (727807) 727807..728286 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
G9W36_RS03495 (728372) 728372..728605 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
G9W36_RS03500 (728608) 728608..728922 + 315 WP_001160966.1 CcdB family protein Toxin
G9W36_RS03505 (728919) 728919..729767 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
G9W36_RS03510 (729774) 729774..730151 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
G9W36_RS03515 (730154) 730154..730975 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
G9W36_RS03520 (730972) 730972..731961 - 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
G9W36_RS03525 (731961) 731961..733247 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T151648 WP_001160966.1 NZ_CP049839:728608-728922 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T151648 NZ_CP065627:c2518551-2518270 [Delftia acidovorans]
ATGGCGATCACTTCCTTCAAGTGCCCGGAAACCCAGCGCCTGTTCACCAGCGGCAAGACCCGCCGCTTCGCCAACATCCA
GAGCGTGGCGGAGCGCAAGCTGGCTCAGCTGGATGCGGCTGTGACACTTGAATTTCTGCGCAGCCCTCCTGGCAATCGCC
TGGAAATGCTCGTGGGAGATCGGCAGGGCCAGCACAGCATCCGCATCAACGACCAGTGGCGCCTCTGCTTTGTCTGGACG
GAAAAGGGAGTGGTTGACGTAGAGATAGTGGATTACCACTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT151648 WP_000125568.1 NZ_CP049839:728372-728605 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT151648 NZ_CP065627:c2518188-2517877 [Delftia acidovorans]
ATGCTTAAAAACGGCATGCGCCCCATACACCCAGGTGAAGTCCTGCGCGAGGACTACCTGAAGCCCATGGACATGAGCGC
CAACGCGCTGGCCAGGCACCTGCACGTTCCCGCCTCGCGCGTGAACGACATCGTGCTGGAGCGGCGCGGCATCACGGCCG
ACACGGCGCTGCGGCTGAGCCGCTTCTTTGGGGGCGACGCCCAGTCCTGGCTGAACCTTCAGACCGCCTACGACCTGCGC
ACGGCAGAGCTGAACGCGGAGTTGCAGGCGTCCGTGAGGCAAGTTCATCCGATGCAGCTGCATACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References