Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 3442429..3443062 Replicon chromosome
Accession NZ_CP049793
Organism Ralstonia solanacearum strain 204

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag G7939_RS16210 Protein ID WP_016725172.1
Coordinates 3442429..3442842 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag G7939_RS16215 Protein ID WP_011002949.1
Coordinates 3442829..3443062 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7939_RS16190 3437811..3439547 + 1737 WP_058908046.1 ABC transporter substrate-binding protein -
G7939_RS16195 3439709..3441205 + 1497 WP_011002945.1 glycerol kinase GlpK -
G7939_RS16200 3441266..3441637 - 372 WP_011002946.1 hypothetical protein -
G7939_RS16205 3441828..3442454 + 627 WP_058908047.1 DUF1415 domain-containing protein -
G7939_RS16210 3442429..3442842 - 414 WP_016725172.1 PIN domain-containing protein Toxin
G7939_RS16215 3442829..3443062 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G7939_RS16220 3443119..3444726 - 1608 WP_058908048.1 glucan biosynthesis protein -
G7939_RS16225 3444921..3446171 + 1251 WP_089190599.1 AGE family epimerase/isomerase -
G7939_RS16230 3446179..3447474 + 1296 WP_011002952.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T151606 WP_016725172.1 NZ_CP049793:c3442842-3442429 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T151606 NZ_CP065615:2644769-2645047 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAATTATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT151606 WP_011002949.1 NZ_CP049793:c3443062-3442829 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT151606 NZ_CP065615:2644506-2644766 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGTGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References