Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 1752974..1754560 Replicon chromosome
Accession NZ_CP049793
Organism Ralstonia solanacearum strain 204

Toxin (Protein)


Gene name hipA Uniprot ID A0A0S4V639
Locus tag G7939_RS08495 Protein ID WP_071507716.1
Coordinates 1752974..1754311 (-) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag G7939_RS08500 Protein ID WP_028853251.1
Coordinates 1754315..1754560 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7939_RS08470 1748619..1749359 + 741 WP_011001390.1 protocatechuate 3,4-dioxygenase subunit beta -
G7939_RS08475 1749356..1750054 + 699 WP_071507713.1 protocatechuate 3,4-dioxygenase subunit alpha -
G7939_RS08480 1750235..1750798 + 564 WP_071654081.1 RNA 2',3'-cyclic phosphodiesterase -
G7939_RS08485 1750821..1751549 - 729 WP_011001393.1 DsbA family protein -
G7939_RS08490 1751775..1752977 - 1203 WP_071507715.1 MFS transporter -
G7939_RS08495 1752974..1754311 - 1338 WP_071507716.1 type II toxin-antitoxin system HipA family toxin Toxin
G7939_RS08500 1754315..1754560 - 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
G7939_RS08505 1754664..1755503 - 840 WP_011001397.1 oxaloacetate decarboxylase -
G7939_RS08515 1755821..1756459 + 639 WP_028853250.1 7-carboxy-7-deazaguanine synthase -
G7939_RS08520 1756527..1757003 + 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
G7939_RS08525 1757023..1757574 + 552 WP_028860693.1 tRNA adenosine(34) deaminase TadA -
G7939_RS08530 1757571..1758497 + 927 WP_028860692.1 muramoyltetrapeptide carboxypeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48738.78 Da        Isoelectric Point: 6.7232

>T151605 WP_071507716.1 NZ_CP049793:c1754311-1752974 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T151605 NZ_CP065615:2399802-2400344 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCAGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGATAGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT151605 WP_028853251.1 NZ_CP049793:c1754560-1754315 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT151605 NZ_CP065615:2399507-2399818 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4V639

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References