Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2051319..2052905 Replicon chromosome
Accession NZ_CP049789
Organism Ralstonia solanacearum strain 202

Toxin (Protein)


Gene name hipA Uniprot ID A0A0S4V639
Locus tag G7969_RS09330 Protein ID WP_071507716.1
Coordinates 2051568..2052905 (+) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag G7969_RS09325 Protein ID WP_028853251.1
Coordinates 2051319..2051564 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7969_RS09295 2047382..2048308 - 927 WP_028860692.1 muramoyltetrapeptide carboxypeptidase -
G7969_RS09300 2048305..2048856 - 552 WP_028860693.1 tRNA adenosine(34) deaminase TadA -
G7969_RS09305 2048876..2049352 - 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
G7969_RS09310 2049420..2050058 - 639 WP_028853250.1 7-carboxy-7-deazaguanine synthase -
G7969_RS09320 2050376..2051215 + 840 WP_011001397.1 oxaloacetate decarboxylase -
G7969_RS09325 2051319..2051564 + 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
G7969_RS09330 2051568..2052905 + 1338 WP_071507716.1 type II toxin-antitoxin system HipA family toxin Toxin
G7969_RS09335 2052902..2054104 + 1203 WP_071507715.1 MFS transporter -
G7969_RS09340 2054330..2055058 + 729 WP_011001393.1 DsbA family protein -
G7969_RS09345 2055081..2055644 - 564 WP_071654081.1 RNA 2',3'-cyclic phosphodiesterase -
G7969_RS09350 2055825..2056523 - 699 WP_071507713.1 protocatechuate 3,4-dioxygenase subunit alpha -
G7969_RS09355 2056520..2057260 - 741 WP_011001390.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48738.78 Da        Isoelectric Point: 6.7232

>T151597 WP_071507716.1 NZ_CP049789:2051568-2052905 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T151597 NZ_CP065614:c132136-131654 [Escherichia coli]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACCCAAGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT151597 WP_028853251.1 NZ_CP049789:2051319-2051564 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT151597 NZ_CP065614:c132429-132127 [Escherichia coli]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4V639

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References