Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 418191..418824 Replicon chromosome
Accession NZ_CP049787
Organism Ralstonia solanacearum strain B2

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag G7968_RS01895 Protein ID WP_016725172.1
Coordinates 418411..418824 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag G7968_RS01890 Protein ID WP_011002949.1
Coordinates 418191..418424 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7968_RS01875 413779..415074 - 1296 WP_011002952.1 MFS transporter -
G7968_RS01880 415082..416332 - 1251 WP_111375179.1 AGE family epimerase/isomerase -
G7968_RS01885 416527..418134 + 1608 WP_165591127.1 glucan biosynthesis protein -
G7968_RS01890 418191..418424 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G7968_RS01895 418411..418824 + 414 WP_016725172.1 PIN domain-containing protein Toxin
G7968_RS01900 418799..419425 - 627 WP_019718918.1 DUF1415 family protein -
G7968_RS01905 419616..419987 + 372 WP_165591128.1 hypothetical protein -
G7968_RS01910 420052..421548 - 1497 WP_011002945.1 glycerol kinase GlpK -
G7968_RS01915 421710..423446 - 1737 WP_019718916.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T151592 WP_016725172.1 NZ_CP049787:418411-418824 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T151592 NZ_CP065613:c3982490-3981948 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGATAGCGCG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT151592 WP_011002949.1 NZ_CP049787:418191-418424 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT151592 NZ_CP065613:c3982785-3982474 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References