Detailed information of TA system TA151576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2409927..2410501
• Replicon: chromosome
• Accession: NZ_CP049786
• Organism: Leclercia sp. 29361

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G7090_RS11485
• Protein ID: WP_106992912.1
• Coordinates: 2410175..2410501 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A6G7TED5
• Locus tag: G7090_RS11480
• Protein ID: WP_059307895.1
• Coordinates: 2409927..2410175 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7090_RS11460	2406031..2407575	+	1545	WP_142489509.1	nitrate reductase subunit beta	-
G7090_RS11465	2407575..2408270	+	696	WP_166182487.1	nitrate reductase molybdenum cofactor assembly chaperone	-
G7090_RS11470	2408267..2408947	+	681	WP_106992911.1	respiratory nitrate reductase subunit gamma	-
G7090_RS11475	2409143..2409592	-	450	WP_166182488.1	hypothetical protein	-
G7090_RS11480	2409927..2410175	+	249	WP_059307895.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G7090_RS11485	2410175..2410501	+	327	WP_106992912.1	endoribonuclease MazF	Toxin
G7090_RS11490	2410530..2411375	-	846	WP_166182489.1	N-hydroxyarylamine O-acetyltransferase	-
G7090_RS11495	2411447..2413102	-	1656	WP_166182490.1	iron ABC transporter permease	-
G7090_RS11500	2413141..2414109	-	969	WP_166182491.1	ABC transporter substrate-binding protein	-
G7090_RS11505	2414106..2414798	-	693	WP_166182492.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11909.66 Da
• Isoelectric Point: 7.8553

>T151576 WP_106992912.1 NZ_CP049786:2410175-2410501 [Leclercia sp. 29361]
MDYIPEAGDIVWLDFDPQSGHEQAGHRPALVISPATYNGRTGLMLCCPLTSQIKNYPFEVVISGSRRNVALADQIKNLDW
RARNAVKKGTATAEELDAVRMRAKVLIG

Nucleotide

• Length: 327 bp

>T151576 NZ_CP065613:660317-660607 [Escherichia coli]
ATGTTACCCGTTTTATGGCTACCATCTGCTCGTGATGATTTGCGCCAGATCGTAGCCTACATAGCTAAAGAAAATCCCGC
GGCAGCACGCAGAATGAAAATTCGAATTGAAACATCCGTTTTGCCGCTCACAGAGCATCCTTACCTGTATCCTCCAAGCG
AGCGAGTTCTTGGTCTTCGTGAAATTGTAACTCATCCTAACTACATCATCCTTTACCGAGTAACAGCATCAAGCATAGAA
GTAGTAAATATTGTGCATTCTCGAAGACAGTATCCAGGCAAAAACAGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9137.39 Da
• Isoelectric Point: 4.4777

>AT151576 WP_059307895.1 NZ_CP049786:2409927-2410175 [Leclercia sp. 29361]
MPQVTVKKWGNSPSVRLPVAVMREAELHVDDVVNVNVDEEGRIILTPVRKDEPTLESLLAAITDDNLHHEVTFGEPQGKE
LL

Nucleotide

• Length: 249 bp

>AT151576 NZ_CP065613:660123-660317 [Escherichia coli]
ATGAGTACAGTCCTTTCCCCAATGGTTTCAGAATTTGAGACAGTTGAGCAAGAAAATAGTTACAACGAATGGCTGCGGGC
CAAAGTAGCAGCAAGCCTCGCGGATCACCGCCCGGCGATTCCGCATGATGAGGTAATGGCGGAAATGGAACAAATTATTG
CCCAAATTGCTGCTGCAACTCACAGAGGTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G7TED5