Detailed information of TA system TA151563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 6937..8074
• Replicon: plasmid unnamed2
• Accession: NZ_CP049777
• Organism: Enterococcus faecalis strain ES-1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: Q54944
• Locus tag: G7X26_RS14235
• Protein ID: WP_001284311.1
• Coordinates: 7211..8074 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: G7X26_RS14230
• Protein ID: WP_000301765.1
• Coordinates: 6937..7209 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7X26_RS14200	2156..2374	+	219	WP_129235162.1	helix-turn-helix transcriptional regulator	-
G7X26_RS14205	2683..2853	+	171	WP_000713594.1	hypothetical protein	-
G7X26_RS14210	2867..3484	+	618	WP_001062589.1	recombinase family protein	-
G7X26_RS14215	3484..5628	+	2145	WP_000108741.1	type IA DNA topoisomerase	-
G7X26_RS14220	5717..6613	+	897	WP_001835294.1	ParA family protein	-
G7X26_RS14225	6711..6920	+	210	WP_000527318.1	peptide-binding protein	-
G7X26_RS14230	6937..7209	+	273	WP_000301765.1	antitoxin	Antitoxin
G7X26_RS14235	7211..8074	+	864	WP_001284311.1	toxin zeta	Toxin
G7X26_RS14240	8248..9588	-	1341	WP_010728931.1	ISL3 family transposase	-
G7X26_RS14245	9810..10229	-	420	WP_016922374.1	fosfomycin resistance bacillithiol transferase FosB3	-
G7X26_RS14255	10766..11503	-	738	WP_050047025.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
G7X26_RS14260	11628..11711	-	84	WP_031929417.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
G7X26_RS14270	12198..12659	-	462	Protein_14	zeta toxin family protein	-
G7X26_RS14515	12639..12875	-	237	Protein_15	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosB3 / erm(B) / aac(6')-aph(2'')	-	1..53121	53121
	-	flank	IS/Tn	-	-	1136..2044	908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32404.02 Da
• Isoelectric Point: 6.9964

>T151563 WP_001284311.1 NZ_CP049777:7211-8074 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T151563 NZ_CP065611:c3713285-3713007 [Escherichia coli]
ATGATTCAAAGGGATATTGAATATTCGGGGCAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT151563 WP_000301765.1 NZ_CP049777:6937-7209 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT151563 NZ_CP065611:c3713548-3713288 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 1GVN
• PDB: 3Q8X

Antitoxin

• AlphaFold DB: A0A829F0A3