151562

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 79148..80285 Replicon plasmid unnamed1
Accession NZ_CP049776
Organism Enterococcus faecalis strain ES-1

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag G7X26_RS14140 Protein ID WP_002332783.1
Coordinates 79148..80011 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag G7X26_RS14145 Protein ID WP_002326825.1
Coordinates 80013..80285 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7X26_RS14105 74817..75554 - 738 WP_002321849.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
G7X26_RS14110 75679..75762 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
G7X26_RS14115 75811..76052 - 242 Protein_85 peptide-binding protein -
G7X26_RS14120 76172..76798 - 627 Protein_86 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
G7X26_RS14125 76874..77554 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
G7X26_RS14130 77588..78094 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
G7X26_RS14135 78391..78708 - 318 WP_002326830.1 hypothetical protein -
G7X26_RS14140 79148..80011 - 864 WP_002332783.1 toxin zeta Toxin
G7X26_RS14145 80013..80285 - 273 WP_002326825.1 antitoxin Antitoxin
G7X26_RS14150 80303..80512 - 210 WP_000527318.1 peptide-binding protein -
G7X26_RS14155 80610..81506 - 897 WP_002326827.1 ParA family protein -
G7X26_RS14160 81609..81869 - 261 Protein_94 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
G7X26_RS14165 82039..82776 - 738 WP_024408862.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
G7X26_RS14170 82901..82984 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
G7X26_RS14180 83416..83787 - 372 WP_002358205.1 hypothetical protein -
G7X26_RS14185 83780..84625 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / erm(B) / dfrG - 1..85096 85096
- inside IS/Tn erm(B) / dfrG - 74817..82776 7959


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T151562 WP_002332783.1 NZ_CP049776:c80011-79148 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T151562 NZ_CP065611:3370326-3370613 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT151562 WP_002326825.1 NZ_CP049776:c80285-80013 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT151562 NZ_CP065611:3370600-3370902 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References