151561

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 68210..68781 Replicon plasmid unnamed1
Accession NZ_CP049776
Organism Enterococcus faecalis strain ES-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A2Z2CGM4
Locus tag G7X26_RS14045 Protein ID WP_002362432.1
Coordinates 68210..68551 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID R3KHK9
Locus tag G7X26_RS14050 Protein ID WP_002362431.1
Coordinates 68551..68781 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7X26_RS14020 63224..63919 - 696 WP_002405176.1 Fic family protein -
G7X26_RS14025 63968..64567 - 600 WP_002405175.1 tyrosine-type recombinase/integrase -
G7X26_RS14035 66293..66895 - 603 WP_002362434.1 Fic family protein -
G7X26_RS14040 67160..68098 - 939 WP_002362433.1 hypothetical protein -
G7X26_RS14045 68210..68551 - 342 WP_002362432.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
G7X26_RS14050 68551..68781 - 231 WP_002362431.1 hypothetical protein Antitoxin
G7X26_RS14055 68985..69605 + 621 WP_002367784.1 recombinase family protein -
G7X26_RS14060 69595..69909 + 315 WP_002367785.1 hypothetical protein -
G7X26_RS14065 70269..70463 + 195 WP_002367787.1 hypothetical protein -
G7X26_RS14070 70475..70666 + 192 WP_002367788.1 hypothetical protein -
G7X26_RS14075 70836..71051 + 216 WP_002367791.1 leucocin A/sakacin P family class II bacteriocin -
G7X26_RS14080 71052..71393 + 342 WP_002367792.1 bacteriocin immunity protein -
G7X26_RS14085 71809..72327 + 519 WP_002367793.1 hypothetical protein -
G7X26_RS14090 72582..72668 + 87 WP_012881081.1 type I toxin-antitoxin system Fst family toxin -
G7X26_RS14095 72925..73221 + 297 WP_002367795.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cat / tet(L) / tet(M) / erm(B) / dfrG - 1..85096 85096


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13244.44 Da        Isoelectric Point: 8.0113

>T151561 WP_002362432.1 NZ_CP049776:c68551-68210 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T151561 NZ_CP065611:3172636-3172809 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8510.42 Da        Isoelectric Point: 4.3855

>AT151561 WP_002362431.1 NZ_CP049776:c68781-68551 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Download         Length: 231 bp

>AT151561 NZ_CP065611:3172312-3172608 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z2CGM4

Antitoxin

Source ID Structure
AlphaFold DB R3KHK9

References