Detailed information of TA system TA151543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 181263..182400
• Replicon: chromosome
• Accession: NZ_CP049775
• Organism: Enterococcus faecalis strain ES-1

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: G7X26_RS00865
• Protein ID: WP_002333003.1
• Coordinates: 181537..182400 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: B3CKC7
• Locus tag: G7X26_RS00860
• Protein ID: WP_002333002.1
• Coordinates: 181263..181535 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7X26_RS00840	177590..177826	-	237	Protein_167	hypothetical protein	-
G7X26_RS00845	178101..179939	+	1839	WP_165548682.1	type IA DNA topoisomerase	-
G7X26_RS00850	180042..180938	+	897	WP_002387620.1	ParA family protein	-
G7X26_RS00855	181036..181245	+	210	WP_000527318.1	peptide-binding protein	-
G7X26_RS00860	181263..181535	+	273	WP_002333002.1	antitoxin	Antitoxin
G7X26_RS00865	181537..182400	+	864	WP_002333003.1	zeta toxin family protein	Toxin
G7X26_RS00870	182522..183019	+	498	Protein_173	DnaJ domain-containing protein	-
G7X26_RS00875	183104..183700	-	597	WP_002326773.1	TetR/AcrR family transcriptional regulator	-
G7X26_RS00880	184011..184892	+	882	WP_002326774.1	ABC transporter ATP-binding protein	-
G7X26_RS00885	184916..186529	+	1614	WP_002333004.1	hypothetical protein	-
G7X26_RS00890	186601..187125	-	525	WP_010717848.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	187167..187847	680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32365.76 Da
• Isoelectric Point: 6.3643

>T151543 WP_002333003.1 NZ_CP049775:181537-182400 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVVVIDNDTFKQQHPNFDELV
KLYEKDVVKHATPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKTYAMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Nucleotide

• Length: 864 bp

>T151543 NZ_CP065607:c3592970-3592752 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.29 Da
• Isoelectric Point: 7.1987

>AT151543 WP_002333002.1 NZ_CP049775:181263-181535 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT151543 NZ_CP065607:c3593370-3592996 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2Z6BWJ3