Detailed information of TA system TA151530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4838457..4839036
• Replicon: chromosome
• Accession: NZ_CP049752
• Organism: Raoultella ornithinolytica strain WM1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: G7Z36_RS22850
• Protein ID: WP_097358154.1
• Coordinates: 4838749..4839036 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: G7Z36_RS22845
• Protein ID: WP_097358155.1
• Coordinates: 4838457..4838762 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Z36_RS22835	4834497..4837763	+	3267	WP_097358157.1	type I restriction endonuclease subunit R	-
G7Z36_RS22840	4837987..4838322	+	336	WP_097358156.1	endoribonuclease SymE	-
G7Z36_RS22845	4838457..4838762	-	306	WP_097358155.1	BrnA antitoxin family protein	Antitoxin
G7Z36_RS22850	4838749..4839036	-	288	WP_097358154.1	BrnT family toxin	Toxin
G7Z36_RS22855	4839153..4839572	-	420	WP_015585224.1	FosA family fosfomycin resistance glutathione transferase	-
G7Z36_RS22860	4839566..4840474	-	909	WP_004857513.1	LysR family transcriptional regulator	-
G7Z36_RS22865	4840562..4841344	+	783	WP_097358153.1	NAD(P)H-dependent oxidoreductase	-
G7Z36_RS22870	4841492..4842070	+	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
G7Z36_RS22875	4842214..4843026	+	813	WP_004857492.1	winged helix-turn-helix domain-containing protein	-
G7Z36_RS22880	4843023..4843541	+	519	WP_004857491.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11136.59 Da
• Isoelectric Point: 7.4685

>T151530 WP_097358154.1 NZ_CP049752:c4839036-4838749 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRQERYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T151530 NZ_CP065607:c1294061-1293654 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11667.19 Da
• Isoelectric Point: 9.4429

>AT151530 WP_097358155.1 NZ_CP049752:c4838762-4838457 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSDAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIREQNKKS

Nucleotide

• Length: 306 bp

>AT151530 NZ_CP065607:c1294308-1294042 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA