Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4261461..4262080 Replicon chromosome
Accession NZ_CP049752
Organism Raoultella ornithinolytica strain WM1

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag G7Z36_RS20165 Protein ID WP_004858783.1
Coordinates 4261862..4262080 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag G7Z36_RS20160 Protein ID WP_004858785.1
Coordinates 4261461..4261835 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7Z36_RS20150 4256571..4257764 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G7Z36_RS20155 4257787..4260933 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit -
G7Z36_RS20160 4261461..4261835 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
G7Z36_RS20165 4261862..4262080 + 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
G7Z36_RS20170 4262232..4262798 + 567 WP_054828907.1 maltose O-acetyltransferase -
G7Z36_RS20175 4262898..4263368 + 471 WP_004858780.1 YlaC family protein -
G7Z36_RS20180 4263343..4264794 - 1452 WP_165552217.1 PLP-dependent aminotransferase family protein -
G7Z36_RS20185 4264897..4265607 + 711 WP_165552219.1 GNAT family N-acetyltransferase -
G7Z36_RS20190 4265604..4265744 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
G7Z36_RS20195 4265747..4266007 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T151529 WP_004858783.1 NZ_CP049752:4261862-4262080 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T151529 NZ_CP065607:c1148477-1148142 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT151529 WP_004858785.1 NZ_CP049752:4261461-4261835 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT151529 NZ_CP065607:c1148725-1148477 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References