Detailed information of TA system TA151528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2811308..2812911
• Replicon: chromosome
• Accession: NZ_CP049752
• Organism: Raoultella ornithinolytica strain WM1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A1Y6GHD8
• Locus tag: G7Z36_RS13390
• Protein ID: WP_015584335.1
• Coordinates: 2811308..2812639 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: G7Z36_RS13395
• Protein ID: WP_032717448.1
• Coordinates: 2812639..2812911 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Z36_RS13375	2807182..2808096	-	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-
G7Z36_RS13380	2808196..2809254	-	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
G7Z36_RS13385	2809969..2811375	+	1407	WP_137056690.1	glycoside hydrolase family 10 protein	-
G7Z36_RS13390	2811308..2812639	-	1332	WP_015584335.1	type II toxin-antitoxin system HipA family toxin	Toxin
G7Z36_RS13395	2812639..2812911	-	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
G7Z36_RS13400	2813114..2813668	-	555	WP_004861926.1	RNA polymerase sigma factor	-
G7Z36_RS13405	2813674..2814099	-	426	WP_032724724.1	hypothetical protein	-
G7Z36_RS13410	2814231..2815184	-	954	WP_004861921.1	hypothetical protein	-
G7Z36_RS13415	2815398..2816030	-	633	WP_069475943.1	class I SAM-dependent methyltransferase	-
G7Z36_RS13420	2816050..2817291	-	1242	WP_165551897.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2769033..2935672	166639

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49327.66 Da
• Isoelectric Point: 6.7012

>T151528 WP_015584335.1 NZ_CP049752:c2812639-2811308 [Raoultella ornithinolytica]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENAPRGALSWEPLDNHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGEQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSG

Nucleotide

• Length: 1332 bp

>T151528 NZ_CP065607:c269128-268877 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT151528 WP_032717448.1 NZ_CP049752:c2812911-2812639 [Raoultella ornithinolytica]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT151528 NZ_CP065607:c268880-268626 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A1Y6GHD8

Antitoxin

• AlphaFold DB: A0A808YAX5