Detailed information of TA system TA151527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2798611..2799362
• Replicon: chromosome
• Accession: NZ_CP049752
• Organism: Raoultella ornithinolytica strain WM1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: G7Z36_RS13335
• Protein ID: WP_044351088.1
• Coordinates: 2798880..2799362 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: G7Z36_RS13330
• Protein ID: WP_015584326.1
• Coordinates: 2798611..2798889 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Z36_RS13320	2795400..2795897	+	498	WP_048023692.1	hypothetical protein	-
G7Z36_RS13325	2796055..2797980	-	1926	WP_099843528.1	C1 family peptidase	-
G7Z36_RS13330	2798611..2798889	+	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
G7Z36_RS13335	2798880..2799362	+	483	WP_044351088.1	GNAT family N-acetyltransferase	Toxin
G7Z36_RS13340	2800039..2800671	+	633	WP_001567369.1	hypothetical protein	-
G7Z36_RS13345	2800700..2802103	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
G7Z36_RS13350	2802283..2802444	-	162	WP_155075028.1	hypothetical protein	-
G7Z36_RS13355	2803163..2804038	-	876	WP_032717451.1	NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2769033..2935672	166639
	-	flank	IS/Tn	-	-	2800700..2802103	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17612.48 Da
• Isoelectric Point: 9.7381

>T151527 WP_044351088.1 NZ_CP049752:2798880-2799362 [Raoultella ornithinolytica]
MGMRPPEPLSAGHNIAEFCCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVPGAYRRNAP
EAVPVIVLGRLAVDAAWAGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T151527 NZ_CP065607:c268880-268626 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT151527 WP_015584326.1 NZ_CP049752:2798611-2798889 [Raoultella ornithinolytica]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT151527 NZ_CP065607:c269128-268877 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8