Detailed information of TA system TA151516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 67167..67714
• Replicon: chromosome
• Accession: NZ_CP049752
• Organism: Raoultella ornithinolytica strain WM1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: G7Z36_RS00315
• Protein ID: WP_123629131.1
• Coordinates: 67167..67475 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A808XYT1
• Locus tag: G7Z36_RS00320
• Protein ID: WP_004869059.1
• Coordinates: 67478..67714 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Z36_RS00285	62346..62648	-	303	WP_015585486.1	PTS lactose/cellobiose transporter subunit IIA	-
G7Z36_RS00290	62783..64108	-	1326	WP_004869064.1	PTS cellobiose transporter subunit IIC	-
G7Z36_RS00295	64120..64434	-	315	WP_004869063.1	PTS sugar transporter subunit IIB	-
G7Z36_RS00300	64729..65670	+	942	WP_015585487.1	LacI family transcriptional regulator	-
G7Z36_RS00305	65765..66040	-	276	WP_160873538.1	hypothetical protein	-
G7Z36_RS00310	66149..67051	-	903	WP_015585496.1	EamA family transporter	-
G7Z36_RS00315	67167..67475	-	309	WP_123629131.1	CcdB family protein	Toxin
G7Z36_RS00320	67478..67714	-	237	WP_004869059.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G7Z36_RS00325	67820..69253	-	1434	WP_004869058.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
G7Z36_RS00330	69278..70180	-	903	WP_088402359.1	N-acetylmuramic acid 6-phosphate etherase	-
G7Z36_RS00335	70345..71508	-	1164	WP_004869055.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11792.64 Da
• Isoelectric Point: 6.7156

>T151516 WP_123629131.1 NZ_CP049752:c67475-67167 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRIVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Nucleotide

• Length: 309 bp

>T151516 NZ_CP065604:118638-118937 [Aggregatibacter actinomycetemcomitans]
ATGATGATACAAATTAAGACGACTTTTCTTTTTGATGAATGGTTGAAGAAACTCAAAAACCTAAGAGCTAAAGCAAAAAT
CAACGCACGAATTAAACGGTTACAGTTTGGTAATTTCGGTGATTTAAAAAGCGTTAATGACGGCATATTTGAAATGCGGA
TTGATAAAGGTCAGGGGTATCGGGTTTATCTGAAAAATCAAAATGGCATACTTGTTATCCTGCTTTGTGGCGGAGACAAA
TCCACTCAAGAGAAAGATATCAAGAAAGCAAAACAACTTGCACAGGAAATGGGACTATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8898.08 Da
• Isoelectric Point: 5.6579

>AT151516 WP_004869059.1 NZ_CP049752:c67714-67478 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT151516 NZ_CP065604:118934-119230 [Aggregatibacter actinomycetemcomitans]
ATGAGTGAACAATTGAAAGATTTTGATCCTGCAGAATATTTATCTACCGAAGAAGATATTCGGCTTTATTTAAATGAAGT
GCTACAAGAAAATAATATTGAGCTTATTTTGTCTGCGTTAGGCGATATCGCACGAACCAGAAATATGAGCCAAATTGCCC
GCGAAACCGGTATGAGTAGAGAAGGGCTTTATAAAGCCCTGTCCGGTACGGGCAATCCTACCTTTGCCACCGTGATTAAA
GTCATGAAAGCCTTAAATTTACAACTCCAAGTGCAACCTTCCGGTTATGTTTCTTAG

Structures

Antitoxin

• AlphaFold DB: A0A808XYT1