Detailed information of TA system TA151514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1240916..1241833
• Replicon: chromosome
• Accession: NZ_CP049741
• Organism: Bacillus velezensis strain UB2017

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: G7X27_RS06470
• Protein ID: WP_007407256.1
• Coordinates: 1241087..1241833 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: G7X27_RS06465
• Protein ID: WP_003154807.1
• Coordinates: 1240916..1241086 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7X27_RS06430	1236149..1237771	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
G7X27_RS06435	1237784..1238155	+	372	WP_032874603.1	YomQ/XkdW protein, phage-like element PBSX	-
G7X27_RS06440	1238160..1238357	+	198	WP_032874605.1	XkdX family protein	-
G7X27_RS06445	1238414..1239175	+	762	WP_032874607.1	hypothetical protein	-
G7X27_RS06450	1239227..1239490	+	264	WP_003154815.1	hemolysin XhlA family protein	-
G7X27_RS06455	1239504..1239767	+	264	WP_003154813.1	phage holin	-
G7X27_RS06460	1239781..1240659	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
G7X27_RS06465	1240916..1241086	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
G7X27_RS06470	1241087..1241833	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
G7X27_RS06475	1241938..1242936	-	999	WP_032874611.1	inorganic phosphate transporter	-
G7X27_RS06480	1242949..1243566	-	618	WP_032874614.1	DUF47 domain-containing protein	-
G7X27_RS06485	1243852..1245168	-	1317	WP_032874616.1	amino acid permease	-
G7X27_RS06490	1245490..1246440	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1193978..1299685	105707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T151514 WP_007407256.1 NZ_CP049741:c1241833-1241087 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T151514 NZ_CP065602:17291-17641 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT151514 WP_003154807.1 NZ_CP049741:c1241086-1240916 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT151514 NZ_CP065602:17046-17297 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14