Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5328823..5329448 Replicon chromosome
Accession NZ_CP049716
Organism Klebsiella pneumoniae strain 11KM256

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag G7Z27_RS26190 Protein ID WP_002882817.1
Coordinates 5328823..5329206 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag G7Z27_RS26195 Protein ID WP_004150355.1
Coordinates 5329206..5329448 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7Z27_RS26175 5326189..5327091 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
G7Z27_RS26180 5327088..5327723 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
G7Z27_RS26185 5327720..5328649 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
G7Z27_RS26190 5328823..5329206 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
G7Z27_RS26195 5329206..5329448 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
G7Z27_RS26200 5329653..5330570 + 918 WP_002882812.1 alpha/beta hydrolase -
G7Z27_RS26205 5330584..5331525 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
G7Z27_RS26210 5331570..5332007 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
G7Z27_RS26215 5332004..5332864 - 861 WP_002882807.1 virulence factor BrkB family protein -
G7Z27_RS26220 5332858..5333457 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T151452 WP_002882817.1 NZ_CP049716:c5329206-5328823 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T151452 NZ_CP065515:c2217405-2217196 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT151452 WP_004150355.1 NZ_CP049716:c5329448-5329206 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT151452 NZ_CP065515:c2217710-2217417 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCGATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References