Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4746496..4747306 Replicon chromosome
Accession NZ_CP049716
Organism Klebsiella pneumoniae strain 11KM256

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag G7Z27_RS23420 Protein ID WP_002887280.1
Coordinates 4746496..4747029 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag G7Z27_RS23425 Protein ID WP_002887278.1
Coordinates 4747040..4747306 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7Z27_RS23415 4745327..4746448 + 1122 WP_002887282.1 cupin domain-containing protein -
G7Z27_RS23420 4746496..4747029 - 534 WP_002887280.1 GNAT family N-acetyltransferase Toxin
G7Z27_RS23425 4747040..4747306 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
G7Z27_RS23430 4747409..4748842 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
G7Z27_RS23435 4748832..4749515 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
G7Z27_RS23440 4749687..4751072 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
G7Z27_RS23445 4751090..4751434 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T151449 WP_002887280.1 NZ_CP049716:c4747029-4746496 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T151449 NZ_CP065515:1973988-1974083 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT151449 WP_002887278.1 NZ_CP049716:c4747306-4747040 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT151449 NZ_CP065515:c1974170-1974111 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References