Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 644768..645462 Replicon chromosome
Accession NZ_CP049703
Organism Parageobacillus toebii NBRC 107807 strain DSM 14590

Toxin (Protein)


Gene name mazF Uniprot ID A0A6G9IZX1
Locus tag DER53_RS03345 Protein ID WP_062755465.1
Coordinates 644768..645154 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6G9J1F3
Locus tag DER53_RS03350 Protein ID WP_062755467.1
Coordinates 645154..645462 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DER53_RS03335 641102..642106 - 1005 WP_121910071.1 CapA family protein -
DER53_RS03340 643259..644449 + 1191 WP_121910017.1 ISL3 family transposase -
DER53_RS03345 644768..645154 - 387 WP_062755465.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DER53_RS03350 645154..645462 - 309 WP_062755467.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DER53_RS03355 646125..646301 - 177 WP_156482448.1 hypothetical protein -
DER53_RS03360 646677..646957 - 281 Protein_645 helix-turn-helix transcriptional regulator -
DER53_RS03370 647296..648192 - 897 WP_062755470.1 hypothetical protein -
DER53_RS03375 648189..648983 - 795 WP_050367864.1 ImmA/IrrE family metallo-endopeptidase -
DER53_RS03380 648967..649380 - 414 WP_062755473.1 hypothetical protein -
DER53_RS03385 649756..649908 - 153 Protein_649 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 618597..688484 69887


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-119)

Antitoxin

SpoVT-AbrB domain

(26-71)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14275.32 Da        Isoelectric Point: 4.7482

>T151434 WP_062755465.1 NZ_CP049703:c645154-644768 [Parageobacillus toebii NBRC 107807]
MPADVPERGDFVVLNFNPQAGHEQAGRRNAIVLSPKEFNQATGFIAVCPITNQKKGYPFEVDLPKEGISLAGDGYPITGV
VLTDQIKSLDWKTRNLKILKKYNPEDAQIEEIDKIIDECLAKIETYLT

Download         Length: 387 bp

>T151434 NZ_CP065468:25654-25806 [Klebsiella quasipneumoniae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11522.17 Da        Isoelectric Point: 7.2044

>AT151434 WP_062755467.1 NZ_CP049703:c645462-645154 [Parageobacillus toebii NBRC 107807]
MNSKEHLESKINQSKGGDYMSTVVVQKWGNSLGIRIPKDAADKIGIKQGSEMELNVIGNEGIITLKPKRTRKKYTLEELI
SQITPENRHEEIDFGIEGRELI

Download         Length: 309 bp

>AT151434 NZ_CP065468:c25602-25542 [Klebsiella quasipneumoniae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G9IZX1

Antitoxin

Source ID Structure
AlphaFold DB A0A6G9J1F3

References