Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 81735..82378 Replicon plasmid unnamed
Accession NZ_CP049605
Organism Klebsiella pneumoniae strain Kp8701

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag G7L41_RS27125 Protein ID WP_001044770.1
Coordinates 81962..82378 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag G7L41_RS27120 Protein ID WP_001261282.1
Coordinates 81735..81965 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7L41_RS27095 77100..78554 + 1455 WP_071836382.1 EAL domain-containing protein -
G7L41_RS27100 79302..80333 + 1032 WP_004176137.1 IS630-like element ISEc33 family transposase -
G7L41_RS27105 80363..80773 - 411 Protein_91 hypothetical protein -
G7L41_RS27110 80825..81175 - 351 WP_023328272.1 hypothetical protein -
G7L41_RS27115 81364..81778 - 415 Protein_93 hypothetical protein -
G7L41_RS27120 81735..81965 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G7L41_RS27125 81962..82378 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
G7L41_RS27130 82452..84014 + 1563 WP_004206609.1 AAA family ATPase -
G7L41_RS27135 83999..85021 + 1023 WP_165430150.1 DNA helicase UvrD -
G7L41_RS27145 85559..86473 + 915 WP_031592169.1 HNH endonuclease -
G7L41_RS27150 86659..87009 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224442 224442
- flank IS/Tn - - 79302..80333 1031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T151356 WP_001044770.1 NZ_CP049605:81962-82378 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T151356 NZ_CP065445:61237-61386 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT151356 WP_001261282.1 NZ_CP049605:81735-81965 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT151356 NZ_CP065445:c61192-61133 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References