Detailed information of TA system TA151339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 55341..55984
• Replicon: plasmid pSECR18-2341_KPC
• Accession: NZ_CP049601
• Organism: Klebsiella aerogenes strain 18-2341

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: G7033_RS25095
• Protein ID: WP_001044770.1
• Coordinates: 55341..55757 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: G7033_RS25100
• Protein ID: WP_001261282.1
• Coordinates: 55754..55984 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7033_RS25070	52152..52856	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
G7033_RS25075	52906..53016	+	111	Protein_49	ANT(3'') family aminoglycoside nucleotidyltransferase	-
G7033_RS25080	53076..53810	+	735	Protein_50	class D beta-lactamase	-
G7033_RS25085	53826..54523	-	698	Protein_51	IS1 family transposase	-
G7033_RS25090	54557..55267	-	711	WP_004152334.1	ATP-binding protein	-
G7033_RS25095	55341..55757	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
G7033_RS25100	55754..55984	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G7033_RS25105	55941..56402	+	462	WP_072093212.1	hypothetical protein	-
G7033_RS25115	56889..57197	+	309	WP_004152336.1	hypothetical protein	-
G7033_RS25120	57225..57554	+	330	WP_004152337.1	hypothetical protein	-
G7033_RS25125	57580..57978	+	399	WP_004171440.1	hypothetical protein	-
G7033_RS25130	57985..58317	+	333	WP_004152339.1	hypothetical protein	-
G7033_RS25135	58317..59099	+	783	WP_004152340.1	site-specific integrase	-
G7033_RS25140	59991..60221	-	231	WP_011977773.1	hypothetical protein	-
G7033_RS25145	60313..60786	-	474	WP_004152341.1	YkgJ family cysteine cluster protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaOXA-9	-	1..119990	119990
	-	flank	IS/Tn	-	-	60906..62225	1319

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T151339 WP_001044770.1 NZ_CP049601:c55757-55341 [Klebsiella aerogenes]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T151339 NZ_CP065441:3478986-3479354 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT151339 WP_001261282.1 NZ_CP049601:c55984-55754 [Klebsiella aerogenes]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT151339 NZ_CP065441:3478768-3478989 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3