Detailed information of TA system TA151321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1798662..1799257
• Replicon: chromosome
• Accession: NZ_CP049362
• Organism: Alcaligenes ammonioxydans strain HO-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: FE795_RS08280
• Protein ID: WP_003800206.1
• Coordinates: 1798952..1799257 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FE795_RS08275
• Protein ID: WP_003800207.1
• Coordinates: 1798662..1798955 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE795_RS08235	1794761..1795129	+	369	WP_219236044.1	hypothetical protein	-
FE795_RS08240 (FE795_08250)	1795286..1795792	+	507	WP_219236046.1	Rha family transcriptional regulator	-
FE795_RS08245 (FE795_08255)	1795832..1796233	+	402	WP_219236048.1	hypothetical protein	-
FE795_RS08250 (FE795_08260)	1796233..1796550	+	318	WP_219236050.1	hypothetical protein	-
FE795_RS08255 (FE795_08265)	1796586..1796969	+	384	WP_219236052.1	hypothetical protein	-
FE795_RS08260 (FE795_08270)	1796947..1797321	+	375	WP_219236053.1	hypothetical protein	-
FE795_RS08265 (FE795_08275)	1797331..1798074	+	744	WP_219236055.1	Ig-like domain-containing protein	-
FE795_RS08270 (FE795_08280)	1798071..1798655	+	585	WP_219236058.1	DUF6246 family protein	-
FE795_RS08275 (FE795_08285)	1798662..1798955	-	294	WP_003800207.1	putative addiction module antidote protein	Antitoxin
FE795_RS08280 (FE795_08290)	1798952..1799257	-	306	WP_003800206.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FE795_RS08285 (FE795_08295)	1799369..1799947	+	579	WP_131071735.1	hypothetical protein	-
FE795_RS08290 (FE795_08300)	1800006..1802582	+	2577	WP_219236060.1	tape measure protein	-
FE795_RS08295 (FE795_08305)	1802598..1802867	-	270	WP_125277533.1	hypothetical protein	-
FE795_RS08300 (FE795_08310)	1802928..1803392	+	465	WP_219236061.1	hypothetical protein	-
FE795_RS08305 (FE795_08315)	1803389..1803871	+	483	WP_219236063.1	DUF1833 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1770482..1940117	169635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-89)

Antitoxin

• Uncharacterized protein HI1420 (3-94)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11521.25 Da
• Isoelectric Point: 10.3556

>T151321 WP_003800206.1 NZ_CP049362:c1799257-1798952 [Alcaligenes ammonioxydans]
MNTINRTETFSTWLTGLKDLKARAKIVVRIKQAGQGNFGDVKPISDGVWEMRIHFGPGYRLYYAREGRVVYLLLSGGDKS
TQKQDIKTAIAMWKQIQEDQS

Nucleotide

• Length: 306 bp

>T151321 NZ_CP065438:538525-538908 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10310.87 Da
• Isoelectric Point: 4.8359

>AT151321 WP_003800207.1 NZ_CP049362:c1798955-1798662 [Alcaligenes ammonioxydans]
MTTIKTAPFDAAEYLDSEEAIAEYLSAALEEDDPALFLSALADVVKARGMTKMAKDAGVGRESLYKALAPGAKPRYDTVL
KLARAAGVKLTVEPLHA

Nucleotide

• Length: 294 bp

>AT151321 NZ_CP065438:538283-538525 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA