Detailed information of TA system TA151320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 50290..50852
• Replicon: plasmid unnamed3
• Accession: NZ_CP049360
• Organism: Deinococcus wulumuqiensis R12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6R31_RS16465
• Protein ID: WP_017870379.1
• Coordinates: 50290..50634 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6R31_RS16470
• Protein ID: WP_025566632.1
• Coordinates: 50631..50852 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6R31_RS16455	45483..48704	+	3222	WP_017870380.1	type I restriction-modification system endonuclease	-
G6R31_RS16460	48709..50268	-	1560	WP_161617916.1	restriction endonuclease subunit S	-
G6R31_RS16465	50290..50634	-	345	WP_017870379.1	DUF5615 family PIN-like protein	Toxin
G6R31_RS16470	50631..50852	-	222	WP_025566632.1	DUF433 domain-containing protein	Antitoxin
G6R31_RS16475	50921..52396	-	1476	WP_017870377.1	type I restriction-modification system subunit M	-
G6R31_RS16480	53146..53724	-	579	WP_017870375.1	hypothetical protein	-
G6R31_RS16485	53811..54305	-	495	WP_017870374.1	hypothetical protein	-
G6R31_RS16490	54459..54782	-	324	WP_017870373.1	hypothetical protein	-
G6R31_RS16495	54906..55298	-	393	WP_017870372.1	hypothetical protein	-
G6R31_RS16500	55374..55595	-	222	WP_017870371.1	hypothetical protein	-
G6R31_RS16505	55600..55836	-	237	WP_017870370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58965	58965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (7-82)

Antitoxin

• Protein of unknown function DUF433 (7-61)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12688.55 Da
• Isoelectric Point: 4.7627

>T151320 WP_017870379.1 NZ_CP049360:c50634-50290 [Deinococcus wulumuqiensis R12]
MTQPQVYWLDAQLPPALAPWLTHTFGVEAYSAAYLGLRDAEDEVIFQAARQADAIIISKDSDFLERLVRLGPPPRLLYVT
CGNTTKARLTEIFAQHFAAAQQLFLRGEAVVEIA

Nucleotide

• Length: 345 bp

>T151320 NZ_CP065438:c131801-131433 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8051.22 Da
• Isoelectric Point: 4.6506

>AT151320 WP_025566632.1 NZ_CP049360:c50852-50631 [Deinococcus wulumuqiensis R12]
MTALDRITVNPSQCGGRPCIRGMRIRVSDLLDLLGAGATPEEILTDYPDLEREDISAALLWAARYIDHPRLSA

Nucleotide

• Length: 222 bp

>AT151320 NZ_CP065438:c132019-131798 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA