Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 11789..12381 Replicon plasmid pT28R-3
Accession NZ_CP049356
Organism Escherichia coli strain T28R

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag G7B60_RS24210 Protein ID WP_000513532.1
Coordinates 11789..12037 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag G7B60_RS24215 Protein ID WP_000681609.1
Coordinates 12034..12381 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7B60_RS24180 6858..7112 + 255 WP_001230709.1 hypothetical protein -
G7B60_RS24185 7109..7351 + 243 WP_000151456.1 hypothetical protein -
G7B60_RS24190 7488..7820 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
G7B60_RS24195 7867..8742 - 876 WP_032492042.1 class A extended-spectrum beta-lactamase CTX-M-64 -
G7B60_RS24200 8995..10257 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
G7B60_RS24205 11007..11300 + 294 WP_000673428.1 hypothetical protein -
G7B60_RS24210 11789..12037 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
G7B60_RS24215 12034..12381 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
G7B60_RS24220 12469..12651 - 183 WP_001428470.1 hypothetical protein -
G7B60_RS24225 13153..13452 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
G7B60_RS24230 13712..14038 - 327 WP_000206697.1 hypothetical protein -
G7B60_RS24235 14040..14480 - 441 WP_023223352.1 hypothetical protein -
G7B60_RS24240 14500..15207 - 708 WP_000108794.1 hypothetical protein -
G7B60_RS24245 15209..15961 - 753 WP_000797966.1 conjugal transfer protein TraL -
G7B60_RS24250 15979..16380 - 402 WP_021575837.1 hypothetical protein -
G7B60_RS24255 16748..17089 + 342 WP_000730722.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-64 / mcr-1.1 - 1..65023 65023
- flank IS/Tn blaCTX-M-64 - 7867..10257 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T151316 WP_000513532.1 NZ_CP049356:11789-12037 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T151316 NZ_CP065436:4804644-4805054 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT151316 WP_000681609.1 NZ_CP049356:12034-12381 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT151316 NZ_CP065436:4804397-4804663 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References