151315

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6858..7351 Replicon plasmid pT28R-3
Accession NZ_CP049356
Organism Escherichia coli strain T28R

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag G7B60_RS24180 Protein ID WP_001230709.1
Coordinates 6858..7112 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag G7B60_RS24185 Protein ID WP_000151456.1
Coordinates 7109..7351 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7B60_RS24145 2433..2594 + 162 WP_001434039.1 type I toxin-antitoxin system Hok family toxin -
G7B60_RS24150 2743..3339 + 597 WP_000125165.1 hypothetical protein -
G7B60_RS24620 3357..3641 - 285 WP_001162371.1 hypothetical protein -
G7B60_RS24160 3715..4380 - 666 WP_015387337.1 P-loop NTPase -
G7B60_RS24165 4967..5296 + 330 WP_000855119.1 hypothetical protein -
G7B60_RS24170 5700..6215 + 516 WP_001025393.1 J domain-containing protein -
G7B60_RS24175 6319..6861 + 543 WP_001083904.1 hypothetical protein -
G7B60_RS24180 6858..7112 + 255 WP_001230709.1 hypothetical protein Toxin
G7B60_RS24185 7109..7351 + 243 WP_000151456.1 hypothetical protein Antitoxin
G7B60_RS24190 7488..7820 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
G7B60_RS24195 7867..8742 - 876 WP_032492042.1 class A extended-spectrum beta-lactamase CTX-M-64 -
G7B60_RS24200 8995..10257 - 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
G7B60_RS24205 11007..11300 + 294 WP_000673428.1 hypothetical protein -
G7B60_RS24210 11789..12037 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-64 / mcr-1.1 - 1..65023 65023
- flank IS/Tn blaCTX-M-64 - 7867..10257 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T151315 WP_001230709.1 NZ_CP049356:6858-7112 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T151315 NZ_CP065436:4351535-4351882 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT151315 WP_000151456.1 NZ_CP049356:7109-7351 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT151315 NZ_CP065436:4351882-4352181 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References