Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3944096..3944672 Replicon chromosome
Accession NZ_CP049353
Organism Escherichia coli strain T28R

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag G7B60_RS19035 Protein ID WP_001297643.1
Coordinates 3944385..3944672 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag G7B60_RS19030 Protein ID WP_000063156.1
Coordinates 3944096..3944398 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7B60_RS19015 3940692..3942842 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
G7B60_RS19020 3942892..3943095 + 204 WP_000467859.1 YbdD/YjiX family protein -
G7B60_RS19025 3943106..3944062 + 957 WP_001297640.1 GTPase -
G7B60_RS19030 3944096..3944398 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
G7B60_RS19035 3944385..3944672 - 288 WP_001297643.1 BrnT family toxin Toxin
G7B60_RS19040 3944871..3945568 - 698 Protein_3714 IS1 family transposase -
G7B60_RS19045 3945625..3945720 + 96 Protein_3715 dienelactone hydrolase family protein -
G7B60_RS19050 3945866..3947098 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
G7B60_RS19055 3947139..3948419 + 1281 WP_032292030.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T151302 WP_001297643.1 NZ_CP049353:c3944672-3944385 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T151302 NZ_CP065431:c1965059-1964730 [Streptococcus suis]
ATGGAATTTGAGTCTTATTCAGTGATACTTGCTCCAGCAGTGGAGAAGGAATTGGCAGTTATTTACGCCTATTTTTCGGA
ACAGTTCTCAGAAGAAATTGCCAAAAGACGAATTGGGATGATTGTCGAAGCATTAGAGAGTTTGCAAATTTTTCCTGAAC
GTGGTTTTAACGCCGATAACCGTTTTGGTAAGCAGATAGACCCGCCTCATCTAACCCGAGGCTATGTCGTTGGAAAAGAC
TACATCGCCCTCTATCGCGTAGTGAAAAATGAAGTTCGAGTTGGTCATCTTTTTGCGACCAAGAGTGATTATGTGAAGTT
GTTGAAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT151302 WP_000063156.1 NZ_CP049353:c3944398-3944096 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT151302 NZ_CP065431:c1965336-1965049 [Streptococcus suis]
TTGATAACTATAAATAAAGATGCCCAAGTAAATTTTAAAACTGATTCCAGACTTTTAGAAAAAGCCAAGGAAGTATTTGC
TATGAACCAGCTGGATTTGACTGCTGGTTTTAACCTCTTCTTGCAAAATATCGCTGTAAAAAATGAGTTGCCAATTCTGA
CAGAAGAAGAGTTGGAAAGAGAAGAGTTGTTTTTACAGTTACAGAAGGAAATCCAGGAAAACCAACAGGCGATTGAGGCA
GGTCACGGGATCAGCCTAGAAAGTGTCAAAGAGAAGTATGGAATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References